Guinea pig cytomegalovirus GP129/131/133, homologues of human cytomegalovirus UL128/130/131A, are necessary for infection of monocytes and macrophages
- PMID: 24659103
- DOI: 10.1099/vir.0.064527-0
Guinea pig cytomegalovirus GP129/131/133, homologues of human cytomegalovirus UL128/130/131A, are necessary for infection of monocytes and macrophages
Abstract
The GP129, GP131 and GP133 genes of guinea pig cytomegalovirus (GPCMV) are homologues of human cytomegalovirus UL128, UL130 and UL131A, respectively, which are essential for infection of endothelial and epithelial cells, and for viral transmission to leukocytes. Our previous study demonstrated that a GPCMV strain lacking the 1.6 kb locus that contains the GP129, GP131 and GP133 genes had a growth defect in animals. Here, we demonstrated that the WT strain, but not the 1.6 kb-deleted strain, formed capsids in macrophages prepared from the peritoneal fluid. To understand the mechanism, we prepared GPCMV strains defective in each of GP129, GP131 and GP133, and found that they were all essential for the infection of peritoneal, splenic and PBMC-derived macrophages/monocytes, and for expression of immediate-early antigens in the macrophages/monocytes, although they were dispensable for infection of fibroblasts. Monocyte/macrophage tropism could be one of the important determinants for viral dissemination in vivo.
© 2014 The Authors.
Similar articles
-
A Homolog Pentameric Complex Dictates Viral Epithelial Tropism, Pathogenicity and Congenital Infection Rate in Guinea Pig Cytomegalovirus.PLoS Pathog. 2016 Jul 7;12(7):e1005755. doi: 10.1371/journal.ppat.1005755. eCollection 2016 Jul. PLoS Pathog. 2016. PMID: 27387220 Free PMC article.
-
Repair of a Mutation Disrupting the Guinea Pig Cytomegalovirus Pentameric Complex Acquired during Fibroblast Passage Restores Pathogenesis in Immune-Suppressed Guinea Pigs and in the Context of Congenital Infection.J Virol. 2016 Aug 12;90(17):7715-27. doi: 10.1128/JVI.00320-16. Print 2016 Sep 1. J Virol. 2016. PMID: 27307567 Free PMC article.
-
Characteristics and functions of human cytomegalovirus UL128 gene/protein.Acta Virol. 2014;58(2):103-7. doi: 10.4149/av_2014_02_103. Acta Virol. 2014. PMID: 24957713 Review.
-
Characterization of the guinea pig CMV gH/gL/GP129/GP131/GP133 complex in infection and spread.Virology. 2013 Jun 20;441(1):75-84. doi: 10.1016/j.virol.2013.03.008. Epub 2013 Apr 4. Virology. 2013. PMID: 23562482
-
Human cytomegalovirus tropism for endothelial cells: not all endothelial cells are created equal.J Virol. 2007 Mar;81(5):2095-101. doi: 10.1128/JVI.01422-06. Epub 2006 Sep 6. J Virol. 2007. PMID: 16956936 Free PMC article. Review. No abstract available.
Cited by
-
Characterization of the Second Apoptosis Inhibitor Encoded by Guinea Pig Cytomegalovirus.J Virol. 2022 Dec 21;96(24):e0162222. doi: 10.1128/jvi.01622-22. Epub 2022 Dec 6. J Virol. 2022. PMID: 36472439 Free PMC article.
-
Chemokines and phosphatidylserine: New binding partners for apoptotic cell clearance.Front Cell Dev Biol. 2022 Aug 25;10:943590. doi: 10.3389/fcell.2022.943590. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36092729 Free PMC article. No abstract available.
-
Endothelial Cell Infection by Guinea Pig Cytomegalovirus Is a Lytic or Persistent Infection Depending on Tissue Origin but Requires Viral Pentamer Complex and pp65 Tegument Protein.J Virol. 2022 Sep 14;96(17):e0083122. doi: 10.1128/jvi.00831-22. Epub 2022 Aug 24. J Virol. 2022. PMID: 36000848 Free PMC article.
-
Inclusion of the Guinea Pig Cytomegalovirus Pentameric Complex in a Live Virus Vaccine Aids Efficacy against Congenital Infection but Is Not Essential for Improving Maternal and Neonatal Outcomes.Viruses. 2021 Nov 26;13(12):2370. doi: 10.3390/v13122370. Viruses. 2021. PMID: 34960639 Free PMC article.
-
A Fully Protective Congenital CMV Vaccine Requires Neutralizing Antibodies to Viral Pentamer and gB Glycoprotein Complexes but a pp65 T-Cell Response Is Not Necessary.Viruses. 2021 Jul 27;13(8):1467. doi: 10.3390/v13081467. Viruses. 2021. PMID: 34452332 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources