[3H-thymidine and 3H-uridine incorporation into the heart cells of the freshwater crayfish Astacus astacus and the swimming crab Portunus holsatus]
- PMID: 2469240
[3H-thymidine and 3H-uridine incorporation into the heart cells of the freshwater crayfish Astacus astacus and the swimming crab Portunus holsatus]
Abstract
DNA and RNA syntheses in the heart cells of two decapod species were investigated with the aid of electron microscopic autoradiography. Isotopes were injected in the cavity of adult animals 4 hours before fixation. 3H-thymidine labeling was found in several satellite cell nuclei and in some particular epicardial cell nuclei. None of myonuclei was labeled. 3H-uridine incorporated in all the nuclei of muscle fibers. Satellite cells were labeled with 3H-uridine very slightly, if at all. Such a peculiarity of biosynthetic processes in the decapod heart satellite cell suggests their myoblastic nature similar to that of satellite cells of somatic muscles. The active 3H-thymidine uptake by the heart satellite cells of adult animals may be accounted for by the permanent growth of the decapods through their whole life span.
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