Separation and identification of anthocyanin extracted from mulberry fruit and the pigment binding properties toward human serum albumin
- PMID: 24930424
- DOI: 10.1021/jf500705s
Separation and identification of anthocyanin extracted from mulberry fruit and the pigment binding properties toward human serum albumin
Abstract
Purple pigments were isolated from mulberry extracts using preparative high-speed countercurrent chromatography (HSCCC) and identified by ESI-MS/MS and high performance liquid chromatography (HPLC) techniques. The solvent system containing methyl tert-butyl ether, 1-butanol, acetonitrile, water, and trifluoroacetic acid (10:30:10:50:0.05; %, v/v) was developed in order to separate anthocyanins with different polarities. Cyanidin 3-O-(6″-O-α-rhamnopyranosyl-β-galactopyranoside) (also known as keracyanin) is the major component present in mulberry (41.3%). Other isolated pigments are cyanidin 3-O-(6″-O-α-rhamnopyranosyl-β-glucopyranoside) and petunidin 3-O-β-glucopyranoside. The binding characteristics of keracyanin with human serum albumin (HSA) were investigated by fluorescence and circular dichroism (CD) spectroscopy. Spectroscopic analysis reveals that HSA fluorescence quenched by keracyanin follows a static mode. Binding of keracyanin to HSA mainly depends on van der Waals force or H-bonds with average binding distance of 2.82 nm. The results from synchronous fluorescence, three-dimensional fluorescence, and CD spectra show that adaptive structure rearrangement and decrease of α-helical structure occur in the presence of keracyanin.
Similar articles
-
Large-scale isolation of high-purity anthocyanin monomers from mulberry fruits by combined chromatographic techniques.J Sep Sci. 2017 Sep;40(17):3506-3512. doi: 10.1002/jssc.201700471. Epub 2017 Aug 14. J Sep Sci. 2017. PMID: 28681407
-
Rapid separation of cyanidin-3-glucoside and cyanidin-3-rutinoside from crude mulberry extract using high-performance countercurrent chromatography and establishment of a volumetric scale-up process.J Sep Sci. 2015 Jun;38(11):1828-36. doi: 10.1002/jssc.201500076. Epub 2015 May 15. J Sep Sci. 2015. PMID: 25800228
-
Preparative isolation and purification of anthocyanins from purple sweet potato by high-speed counter-current chromatography.J Sep Sci. 2009 Jun;32(12):2146-51. doi: 10.1002/jssc.200900038. J Sep Sci. 2009. PMID: 19479751
-
Isolation of two anthocyanin sambubiosides from bilberry (Vaccinium myrtillus) by high-speed counter-current chromatography.J Chromatogr A. 2004 Aug 6;1045(1-2):59-63. doi: 10.1016/j.chroma.2004.06.017. J Chromatogr A. 2004. PMID: 15378879
-
Characterization of anthocyanins from the fruits of baguaçu (Eugenia umbelliflora Berg).J Agric Food Chem. 2003 Aug 27;51(18):5450-4. doi: 10.1021/jf030014z. J Agric Food Chem. 2003. PMID: 12926896
Cited by
-
The Darker the Better: Identification of Chemotype Profile in Soroses of Local and Introduced Mulberry Varieties with Respect to the Colour Type.Foods. 2023 Oct 31;12(21):3985. doi: 10.3390/foods12213985. Foods. 2023. PMID: 37959104 Free PMC article.
-
Exploring the Impact of Cyanidin-3-Glucoside on Inflammatory Bowel Diseases: Investigating New Mechanisms for Emerging Interventions.Int J Mol Sci. 2023 May 28;24(11):9399. doi: 10.3390/ijms24119399. Int J Mol Sci. 2023. PMID: 37298350 Free PMC article. Review.
-
Assessing the Nutritional-Value-Based Therapeutic Potentials and Non-Destructive Approaches for Mulberry Fruit Assessment: An Overview.Comput Intell Neurosci. 2022 Mar 24;2022:6531483. doi: 10.1155/2022/6531483. eCollection 2022. Comput Intell Neurosci. 2022. Retraction in: Comput Intell Neurosci. 2023 Sep 20;2023:9860875. doi: 10.1155/2023/9860875. PMID: 35371246 Free PMC article. Retracted. Review.
-
Insights into the Binding of Dietary Phenolic Compounds to Human Serum Albumin and Food-Drug Interactions.Pharmaceutics. 2020 Nov 21;12(11):1123. doi: 10.3390/pharmaceutics12111123. Pharmaceutics. 2020. PMID: 33233356 Free PMC article. Review.
-
Novel Albumin Nanoparticle Enhanced the Anti-Insulin-Resistant-Hepatoma Activity of Metformin.Int J Nanomedicine. 2020 Jul 23;15:5203-5215. doi: 10.2147/IJN.S253094. eCollection 2020. Int J Nanomedicine. 2020. PMID: 32801686 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
