A T-type Ca2+ current underlies low-threshold Ca2+ potentials in cells of the cat and rat lateral geniculate nucleus
- PMID: 2557441
- PMCID: PMC1189115
- DOI: 10.1113/jphysiol.1989.sp017668
A T-type Ca2+ current underlies low-threshold Ca2+ potentials in cells of the cat and rat lateral geniculate nucleus
Abstract
1. The characteristics of a transient inward Ca2+ current (IT) underlying low-threshold Ca2+ potentials were studied in projection cells of the cat and rat dorsal lateral geniculate nucleus (LGN) in vitro using the single-electrode voltage-clamp technique. 2. In cat LGN slices perfused at 25 degrees C with a solution which included 1 mM-Ca2+ and 3 mM-Mg2+, IT could be evoked by depolarizing voltage steps to -55 mV from a holding potential (Vh) of -95 mV and was abolished by reducing [Ca2+]o from 1 to 0.1 mM. IT was also blocked by 8 mM-Mg2+ and 500 microM-Ni2+, but 500 microM-Cd2+ was a significantly less effective antagonist. 3. The inactivation of IT, which occurred at Vh positive to -65 mV, was removed as Vh approached -100 mV. The process of inactivation removal was also time dependent, with 800-1000 ms needed for total removal. Activation curves for IT showed a threshold of -70 mV and illustrated that IT was extremely voltage sensitive over the voltage range from -65 to -55 mV. 4. The decay phase of IT followed a single-exponential time course with a time constant of decay which was voltage sensitive and ranged from 20 to 100 ms. The mean peak conductance increase associated with IT was 8.4 nS (+/-0.9, S.E.M.). 5. In more 'physiological' conditions (35 degrees C and 1.5 mM-Ca2+, 1 mM-Mg2+) the voltage dependence of activation and inactivation were unaffected. However, the development and decay of IT proceeded more rapidly and only 500-600 ms were needed for total removal of inactivation. Under these conditions, the use of voltage ramps showed that depolarization rates of greater than 30 mV/s were necessary for IT activation. 6. The use of multiple voltage-step protocols illustrated that the process of inactivation removal was rapidly reversed by brief returns to a Vh of -50 mV. Furthermore, any delay in IT activation, once the LGN cell membrane potential was in the IT activation range, resulted in a current of reduced amplitude. 7. Although IT in rat LGN cells was briefer and had a shorter latency to peak, it was otherwise similar to that seen in cat LGN cells. 8. The characteristics of IT are very similar to those of the T-type Ca2+ currents of other excitable membranes. The properties of IT are discussed with respect to its role in generating the low-threshold Ca2+ potentials which are central to the oscillatory behaviour of thalamic projection cells.
Similar articles
-
Mechanisms for signal transformation in lemniscal auditory thalamus.J Neurophysiol. 1996 Dec;76(6):3597-608. doi: 10.1152/jn.1996.76.6.3597. J Neurophysiol. 1996. PMID: 8985860
-
Prevention of Ca(2+)-mediated action potentials in GABAergic local circuit neurones of rat thalamus by a transient K+ current.J Physiol. 1994 Aug 1;478 Pt 3(Pt 3):403-22. doi: 10.1113/jphysiol.1994.sp020261. J Physiol. 1994. PMID: 7965855 Free PMC article.
-
The ventral and dorsal lateral geniculate nucleus of the rat: intracellular recordings in vitro.J Physiol. 1987 Mar;384:587-601. doi: 10.1113/jphysiol.1987.sp016471. J Physiol. 1987. PMID: 3309264 Free PMC article. Review.
-
Pharmacological and anatomical separation of calcium currents in rat dentate granule neurones in vitro.J Physiol. 1989 May;412:93-112. doi: 10.1113/jphysiol.1989.sp017605. J Physiol. 1989. PMID: 2557433 Free PMC article.
-
Identification of two calcium currents in acutely dissociated neurons from the rat lateral geniculate nucleus.J Neurophysiol. 1989 Jun;61(6):1270-83. doi: 10.1152/jn.1989.61.6.1270. J Neurophysiol. 1989. PMID: 2501459
Cited by
-
T-type calcium channels as therapeutic targets in essential tremor and Parkinson's disease.Ann Clin Transl Neurol. 2023 Apr;10(4):462-483. doi: 10.1002/acn3.51735. Epub 2023 Feb 4. Ann Clin Transl Neurol. 2023. PMID: 36738196 Free PMC article. Review.
-
Synthesis of Pseudellone Analogs and Characterization as Novel T-type Calcium Channel Blockers.Mar Drugs. 2018 Nov 28;16(12):475. doi: 10.3390/md16120475. Mar Drugs. 2018. PMID: 30487473 Free PMC article.
-
Selective Blockade of T-Type Ca2+ Channels is Protective Against Alcohol-Withdrawal Induced Seizure and Mortality.Alcohol Alcohol. 2018 Sep 1;53(5):526-531. doi: 10.1093/alcalc/agy042. Alcohol Alcohol. 2018. PMID: 29912275 Free PMC article.
-
Inhibitory peptidergic modulation of C. elegans serotonin neurons is gated by T-type calcium channels.Elife. 2017 Feb 6;6:e22771. doi: 10.7554/eLife.22771. Elife. 2017. PMID: 28165324 Free PMC article.
-
T-type calcium channels contribute to NMDA receptor independent synaptic plasticity in hippocampal regular-spiking oriens-alveus interneurons.J Physiol. 2017 Jun 1;595(11):3449-3458. doi: 10.1113/JP273695. Epub 2017 Mar 22. J Physiol. 2017. PMID: 28134447 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous