Germ-line transmission of a planned alteration made in a hypoxanthine phosphoribosyltransferase gene by homologous recombination in embryonic stem cells
- PMID: 2573070
- PMCID: PMC298403
- DOI: 10.1073/pnas.86.22.8927
Germ-line transmission of a planned alteration made in a hypoxanthine phosphoribosyltransferase gene by homologous recombination in embryonic stem cells
Abstract
Embryonic stem cells (derived from 129/Ola mice) containing a mutant hypoxanthine phosphoribosyltransferase gene that had been corrected in vitro in a planned manner by homologous recombination were injected into blastocysts obtained from C57BL/6J mice. The injected blastocysts were introduced into pseudopregnant female mice to complete their development. Eleven surviving pups were obtained. Nine were chimeras: six males and three females. Two of the males transmitted the embryonic stem cell genome containing the alteration in the hypoxanthine phosphoribosyltransferase gene to their offspring at high frequencies. These experiments demonstrate that a preplanned alteration in a chosen gene can be made in the germ line of an experimental animal by homologous recombination in an embryonic stem cell.
Similar articles
-
Targeted mutation of the Hprt gene in mouse embryonic stem cells.Proc Natl Acad Sci U S A. 1988 Nov;85(22):8583-7. doi: 10.1073/pnas.85.22.8583. Proc Natl Acad Sci U S A. 1988. PMID: 3186749 Free PMC article.
-
Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells.Cell. 1989 Jan 27;56(2):313-21. doi: 10.1016/0092-8674(89)90905-7. Cell. 1989. PMID: 2912572
-
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.Cell. 1987 Nov 6;51(3):503-12. doi: 10.1016/0092-8674(87)90646-5. Cell. 1987. PMID: 2822260
-
Altering the genome by homologous recombination.Science. 1989 Jun 16;244(4910):1288-92. doi: 10.1126/science.2660260. Science. 1989. PMID: 2660260 Review.
-
Gene targeting in murine embryonic stem cells: introduction of specific alterations into the mammalian genome.Genet Anal Tech Appl. 1990 Dec;7(8):219-27. doi: 10.1016/0735-0651(90)90004-y. Genet Anal Tech Appl. 1990. PMID: 2091698 Review.
Cited by
-
Tools for Cre-mediated conditional deletion of floxed alleles from developing cerebellar Purkinje cells.bioRxiv [Preprint]. 2024 Mar 30:2024.03.28.587263. doi: 10.1101/2024.03.28.587263. bioRxiv. 2024. Update in: eNeuro. 2024 Jun 3;11(6):ENEURO.0149-24.2024. doi: 10.1523/ENEURO.0149-24.2024. PMID: 38585758 Free PMC article. Updated. Preprint.
-
Defining Gene Function in Spermatogonial Stem Cells Through Conditional Knockout Approaches.Methods Mol Biol. 2023;2656:261-307. doi: 10.1007/978-1-0716-3139-3_15. Methods Mol Biol. 2023. PMID: 37249877
-
Transgenesis and Genome Engineering: A Historical Review.Methods Mol Biol. 2023;2631:1-32. doi: 10.1007/978-1-0716-2990-1_1. Methods Mol Biol. 2023. PMID: 36995662
-
- Invited Review - Gene-editing techniques and their applications in livestock and beyond.Anim Biosci. 2023 Feb;36(2):333-338. doi: 10.5713/ab.22.0383. Epub 2023 Jan 10. Anim Biosci. 2023. PMID: 36634662 Free PMC article.
-
Behind the developing brains and beating hearts of stem cell-derived embryo models.Open Biol. 2023 Jan;13(1):220325. doi: 10.1098/rsob.220325. Epub 2023 Jan 11. Open Biol. 2023. PMID: 36630196 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical