Measurement of oxidatively induced DNA damage and its repair, by mass spectrometric techniques
- PMID: 25812590
- DOI: 10.3109/10715762.2015.1014814
Measurement of oxidatively induced DNA damage and its repair, by mass spectrometric techniques
Abstract
Oxidatively induced damage caused by free radicals and other DNA-damaging agents generate a plethora of products in the DNA of living organisms. There is mounting evidence for the involvement of this type of damage in the etiology of numerous diseases including carcinogenesis. For a thorough understanding of the mechanisms, cellular repair, and biological consequences of DNA damage, accurate measurement of resulting products must be achieved. There are various analytical techniques, with their own advantages and drawbacks, which can be used for this purpose. Mass spectrometric techniques with isotope dilution, which include gas chromatography (GC) and liquid chromatography (LC), provide structural elucidation of products and ascertain accurate quantification, which are absolutely necessary for reliable measurement. Both gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS), in single or tandem versions, have been used for the measurement of numerous DNA products such as sugar and base lesions, 8,5'-cyclopurine-2'-deoxynucleosides, base-base tandem lesions, and DNA-protein crosslinks, in vitro and in vivo. This article reviews these techniques and their applications in the measurement of oxidatively induced DNA damage and its repair.
Keywords: 8,5’-cyclopurine-2’-deoxynucleosides; DNA base damage; DNA sugar damage; free radicals; gas chromatography-mass spectrometry; hydroxyl radical; isotope dilution; liquid chromatography-mass spectrometry.
Similar articles
-
Identification and quantification of human DNA repair protein NEIL1 by liquid chromatography/isotope-dilution tandem mass spectrometry.J Proteome Res. 2013 Feb 1;12(2):1049-61. doi: 10.1021/pr301037t. Epub 2013 Jan 11. J Proteome Res. 2013. PMID: 23268652
-
Mechanisms of free radical-induced damage to DNA.Free Radic Res. 2012 Apr;46(4):382-419. doi: 10.3109/10715762.2011.653969. Free Radic Res. 2012. PMID: 22276778 Review.
-
Measurement of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines in DNA in vivo by liquid chromatography/isotope-dilution tandem mass spectrometry.Biochem Biophys Res Commun. 2009 Sep 4;386(4):656-60. doi: 10.1016/j.bbrc.2009.06.107. Epub 2009 Jun 23. Biochem Biophys Res Commun. 2009. PMID: 19559005
-
Lymphoblasts of women with BRCA1 mutations are deficient in cellular repair of 8,5'-Cyclopurine-2'-deoxynucleosides and 8-hydroxy-2'-deoxyguanosine.Biochemistry. 2007 Mar 6;46(9):2488-96. doi: 10.1021/bi062022p. Epub 2007 Feb 9. Biochemistry. 2007. PMID: 17288454
-
Free radical-induced damage to DNA: mechanisms and measurement.Free Radic Biol Med. 2002 Jun 1;32(11):1102-15. doi: 10.1016/s0891-5849(02)00826-2. Free Radic Biol Med. 2002. PMID: 12031895 Review.
Cited by
-
The Effect of 8,5'-Cyclo 2'-deoxyadenosine on the Activity of 10-23 DNAzyme: Experimental and Theoretical Study.Int J Mol Sci. 2024 Feb 21;25(5):2519. doi: 10.3390/ijms25052519. Int J Mol Sci. 2024. PMID: 38473767 Free PMC article.
-
The Effects of Particle LET and Fluence on the Complexity and Frequency of Clustered DNA Damage.DNA (Basel). 2024 Mar;4(1):34-51. doi: 10.3390/dna4010002. Epub 2024 Jan 5. DNA (Basel). 2024. PMID: 38282954 Free PMC article.
-
Understanding mechanisms of antioxidant action in health and disease.Nat Rev Mol Cell Biol. 2024 Jan;25(1):13-33. doi: 10.1038/s41580-023-00645-4. Epub 2023 Sep 15. Nat Rev Mol Cell Biol. 2024. PMID: 37714962 Review.
-
Detection of Oxidatively Modified Base Lesion(s) in Defined DNA Sequences by FLARE Quantitative PCR.Methods Mol Biol. 2023;2701:115-134. doi: 10.1007/978-1-0716-3373-1_7. Methods Mol Biol. 2023. PMID: 37574478
-
Protective Effects of Micronutrient Supplements, Phytochemicals and Phytochemical-Rich Beverages and Foods Against DNA Damage in Humans: A Systematic Review of Randomized Controlled Trials and Prospective Studies.Adv Nutr. 2023 Nov;14(6):1337-1358. doi: 10.1016/j.advnut.2023.08.004. Epub 2023 Aug 18. Adv Nutr. 2023. PMID: 37573943 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous