Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea

doi:10.1128/genomeA.00787-15

. 2015 Aug 13;3(4):e00787-15.

doi: 10.1128/genomeA.00787-15.

Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea

You-Jin Kim¹, Yong-Joon Cho², Dae-Won Kim³, Jeong-Sun Yang¹, Hak Kim¹, SungHan Park¹, Young Woo Han¹, Mi-Ran Yun³, Han Saem Lee¹, A-Reum Kim¹, Deok Rim Heo¹, Joo Ae Kim¹, Su Jin Kim¹, Hee-Dong Jung¹, Namil Kim², Seok-Hwan Yoon², Jeong-Gu Nam¹, Hae Ji Kang¹, Hyang-Min Cheong¹, Joo-Shil Lee⁴, Jongsik Chun⁵, Sung Soon Kim⁶

Affiliations

¹ Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea.
² ChunLab, Inc., Seoul National University, Seoul, South Korea.
³ Division of Biosafety Evaluation and Control, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea.
⁴ Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea.
⁵ ChunLab, Inc., Seoul National University, Seoul, South Korea sungskim63@gmail.com jchun@chunlab.com.
⁶ Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea sungskim63@gmail.com jchun@chunlab.com.

PMID: 26272558
PMCID: PMC4536669
DOI: 10.1128/genomeA.00787-15

Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea

You-Jin Kim et al. Genome Announc. 2015.

. 2015 Aug 13;3(4):e00787-15.

doi: 10.1128/genomeA.00787-15.

Authors

Affiliations

¹ Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea.
² ChunLab, Inc., Seoul National University, Seoul, South Korea.
³ Division of Biosafety Evaluation and Control, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea.
⁴ Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea.
⁵ ChunLab, Inc., Seoul National University, Seoul, South Korea sungskim63@gmail.com jchun@chunlab.com.
⁶ Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea sungskim63@gmail.com jchun@chunlab.com.

PMID: 26272558
PMCID: PMC4536669
DOI: 10.1128/genomeA.00787-15

Abstract

The full genome sequence of a Middle East respiratory syndrome coronavirus (MERS-CoV) was identified from cultured and isolated in Vero cells. The viral genome sequence has high similarity to 53 human MERS-CoVs, ranging from 99.5% to 99.8% at the nucleotide level.

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References

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[1] Coleman CM, Frieman MB. 2014. Coronaviruses: important emerging human pathogens. J Virol 88:5209–5212. doi:10.1128/JVI.03488-13. - DOI - PMC - PubMed

[2] Coleman CM, Frieman MB. 2014. Coronaviruses: important emerging human pathogens. J Virol 88:5209–5212. doi:10.1128/JVI.03488-13. - DOI - PMC - PubMed

[3] Mackay IM, Arden KE. 2015. Middle East respiratory syndrome: an emerging coronavirus infection tracked by the crowd. Virus Res 202:60–88. doi:10.1016/j.virusres.2015.01.021. - DOI - PMC - PubMed

[4] Mackay IM, Arden KE. 2015. Middle East respiratory syndrome: an emerging coronavirus infection tracked by the crowd. Virus Res 202:60–88. doi:10.1016/j.virusres.2015.01.021. - DOI - PMC - PubMed

[5] Cotten M, Lam TT, Watson SJ, Palser AL, Petrova V, Grant P, Pybus OG, Rambaut A, Guan Y, Pillay D, Kellam P, Nastouli E. 2013. Full-genome deep sequencing and phylogenetic analysis of novel human betacoronavirus. Emerg Infect Dis 19:736B–742B. doi:10.3201/eid1905.130057. - DOI - PMC - PubMed

[6] Cotten M, Lam TT, Watson SJ, Palser AL, Petrova V, Grant P, Pybus OG, Rambaut A, Guan Y, Pillay D, Kellam P, Nastouli E. 2013. Full-genome deep sequencing and phylogenetic analysis of novel human betacoronavirus. Emerg Infect Dis 19:736B–742B. doi:10.3201/eid1905.130057. - DOI - PMC - PubMed

[7] Langmead B, Salzberg SL. 2012. Fast gapped-read alignment with Bowtie 2. Nat Methods 9:357–359. doi:10.1038/nmeth.1923. - DOI - PMC - PubMed

[8] Langmead B, Salzberg SL. 2012. Fast gapped-read alignment with Bowtie 2. Nat Methods 9:357–359. doi:10.1038/nmeth.1923. - DOI - PMC - PubMed

[9] Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. doi:10.1093/molbev/mst197. - DOI - PMC - PubMed

[10] Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. doi:10.1093/molbev/mst197. - DOI - PMC - PubMed

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Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea

Affiliations

Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea

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