[Advance in Deep Sequencing of Small RNAs for Virus Identification and Discovery]
- PMID: 26524920
[Advance in Deep Sequencing of Small RNAs for Virus Identification and Discovery]
Abstract
Small RNAs (sRNA) are produced abundantly in either plants or animals and function in regulating gene expression or in defense against virus infection. Deep sequencing of small RNAs is an emerging technology in virus identification and de novo assembly of virus genomes and is demonstrated to be an effective method to discover new viruses and monitor virus variation. A significant number of viruses from plants, invertebrates and human cells has been successfully identified using this technology. In this paper, we summarized the principle, operation process and latest advances of sRNA deep sequencing We also showed the feasibility of sRNA deep sequencing by bioinformatic analysis using sRNA deep sequencing dataset public available for the detection of viruses.
Similar articles
-
VirusDetect: An automated pipeline for efficient virus discovery using deep sequencing of small RNAs.Virology. 2017 Jan;500:130-138. doi: 10.1016/j.virol.2016.10.017. Epub 2016 Nov 5. Virology. 2017. PMID: 27825033
-
New virus discovery by deep sequencing of small RNAs.Methods Mol Biol. 2012;883:177-91. doi: 10.1007/978-1-61779-839-9_14. Methods Mol Biol. 2012. PMID: 22589134
-
Deep sequencing of small RNAs in tomato for virus and viroid identification and strain differentiation.PLoS One. 2012;7(5):e37127. doi: 10.1371/journal.pone.0037127. Epub 2012 May 18. PLoS One. 2012. PMID: 22623984 Free PMC article.
-
Accelerating Discovery and Functional Analysis of Small RNAs with New Technologies.Annu Rev Genet. 2015;49:367-94. doi: 10.1146/annurev-genet-112414-054804. Epub 2015 Oct 14. Annu Rev Genet. 2015. PMID: 26473381 Review.
-
Non-coding RNAs in virology: an RNA genomics approach.Biotechnol Genet Eng Rev. 2018 Apr;34(1):90-106. doi: 10.1080/02648725.2018.1471642. Epub 2018 Jun 4. Biotechnol Genet Eng Rev. 2018. PMID: 29865927 Review.