COPI selectively drives maturation of the early Golgi
- PMID: 26709839
- PMCID: PMC4758959
- DOI: 10.7554/eLife.13232
COPI selectively drives maturation of the early Golgi
Abstract
COPI coated vesicles carry material between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous. Previous studies of thermosensitive yeast COPI mutants yielded the surprising conclusion that COPI was dispensable both for the secretion of certain proteins and for Golgi cisternal maturation. To revisit these issues, we optimized the anchor-away method, which allows peripheral membrane proteins such as COPI to be sequestered rapidly by adding rapamycin. Video fluorescence microscopy revealed that COPI inactivation causes an early Golgi protein to remain in place while late Golgi proteins undergo cycles of arrival and departure. These dynamics generate partially functional hybrid Golgi structures that contain both early and late Golgi proteins, explaining how secretion can persist when COPI has been inactivated. Our findings suggest that cisternal maturation involves a COPI-dependent pathway that recycles early Golgi proteins, followed by multiple COPI-independent pathways that recycle late Golgi proteins.
Keywords: COPI; Golgi; Kex2; S. cerevisiae; Sec7; cell biology; cisternal maturation.
Conflict of interest statement
The authors declare that no competing interests exist.
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References
-
- Abazeed ME, Fuller RS. Yeast Golgi-localized, gamma-ear-containing, ADP-ribosylation factor-binding proteins are but adaptor protein-1 is not required for cell-free transport of membrane proteins from the trans-Golgi network to the prevacuolar compartment. Molecular Biology of the Cell. 2008;19:4826–4836. doi: 10.1091/mbc.E07-05-0442. - DOI - PMC - PubMed
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