Review
doi: 10.1016/0166-0934(89)90031-1.
The polymerase chain reaction, a review of the practical limitations for human immunodeficiency virus diagnosis
Affiliations
- PMID: 2674182
- DOI: 10.1016/0166-0934(89)90031-1
Item in Clipboard
Review
The polymerase chain reaction, a review of the practical limitations for human immunodeficiency virus diagnosis
J Virol Methods.
1989 Aug.
Abstract
The polymerase chain reaction (PCR) is a powerful method for the in vitro amplification of specific nucleic acid sequences. As very small amounts of a virus genome can be detected it has obvious diagnostic applications. The background to the reaction and its use for human immunodeficiency virus (HIV) detection are described. The problems likely to be encountered in using PCR as a diagnostic assay (false positives and negatives) and the practical measures which can be taken to overcome them are discussed.
Similar articles
-
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells.Science. 1988 Jan 15;239(4837):295-7. doi: 10.1126/science.3336784. Science. 1988. PMID: 3336784
-
[Polymerase chain reaction for the diagnostic identification of HIV infection].Medicina (Firenze). 1989 Jan-Mar;9(1):41-3. Medicina (Firenze). 1989. PMID: 2739527 Italian.
-
Analysis of human immunodeficiency virus and cytomegalovirus infection by polymerase chain reaction in the acquired immunodeficiency syndrome. An autopsy study.Arch Pathol Lab Med. 1989 Nov;113(11):1239-44. Arch Pathol Lab Med. 1989. PMID: 2554843
-
Nucleic acid amplification in vitro: detection of sequences with low copy numbers and application to diagnosis of human immunodeficiency virus type 1 infection.Clin Microbiol Rev. 1989 Apr;2(2):217-26. doi: 10.1128/CMR.2.2.217. Clin Microbiol Rev. 1989. PMID: 2650862 Free PMC article. Review.
-
The polymerase chain reaction: a new tool for the understanding and diagnosis of HIV-1 infection at the molecular level.Mol Cell Probes. 1991 Aug;5(4):241-59. doi: 10.1016/0890-8508(91)90046-m. Mol Cell Probes. 1991. PMID: 1791846 Review.
Cited by
-
Automated extraction of viral-pathogen RNA and DNA for high-throughput quantitative real-time PCR.J Clin Microbiol. 2005 Nov;43(11):5541-6. doi: 10.1128/JCM.43.11.5541-5546.2005. J Clin Microbiol. 2005. PMID: 16272483 Free PMC article.
-
Fully automated detection of hepatitis C virus RNA in serum and whole-blood samples.Clin Diagn Lab Immunol. 2002 Nov;9(6):1385-8. doi: 10.1128/cdli.9.6.1385-1388.2002. Clin Diagn Lab Immunol. 2002. PMID: 12414781 Free PMC article.
-
Evaluation of an automated sample preparation protocol for quantitative detection of hepatitis C virus RNA.J Clin Microbiol. 2002 Apr;40(4):1447-50. doi: 10.1128/JCM.40.4.1447-1450.2002. J Clin Microbiol. 2002. PMID: 11923371 Free PMC article.
-
Detection of Mycoplasma pneumoniae in spiked clinical samples by nucleic acid sequence-based amplification.J Clin Microbiol. 2002 Apr;40(4):1339-45. doi: 10.1128/JCM.40.4.1339-1345.2002. J Clin Microbiol. 2002. PMID: 11923354 Free PMC article.
-
Detection and confirmation of Mycoplasma pneumoniae in urogenital specimens by PCR.J Clin Microbiol. 1998 Jan;36(1):277-80. doi: 10.1128/JCM.36.1.277-280.1998. J Clin Microbiol. 1998. PMID: 9431966 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
