doi: 10.1002/prot.340050403.
Single crystals of bacteriophage T7 RNA polymerase
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- PMID: 2678097
- DOI: 10.1002/prot.340050403
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Single crystals of bacteriophage T7 RNA polymerase
Proteins.
1989.
Abstract
Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 x 0.3 x 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which is enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2(1) and have unit cell parameters a = 114.5 A, b = 139.6 A, c = 125.7 A, and beta = 98.1 degrees. Self-rotation function studies indicate that there are three molecules per asymmetric unit. The crystals diffract to at least 3.0 A resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination.
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