Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jan 18:6:655.
doi: 10.3389/fimmu.2015.00655. eCollection 2015.

Adipose Mesenchymal Stem Cells Isolated after Manual or Water-jet-Assisted Liposuction Display Similar Properties

Claire Bony  1 , Mailys Cren  1 , Sophie Domergue  2 , Karine Toupet  1 , Christian Jorgensen  3 , Danièle Noël  3
Affiliations

Adipose Mesenchymal Stem Cells Isolated after Manual or Water-jet-Assisted Liposuction Display Similar Properties

Claire Bony et al. Front Immunol. .

Abstract

Mesenchymal stem or stromal cells (MSC) are under investigation in many clinical trials for their therapeutic potential in a variety of diseases, including autoimmune and inflammatory disorders. One of the main sources of MSCs is the adipose tissue, which is mainly obtained by manual liposuction using a cannula linked to a syringe. However, in the past years, a number of devices for fat liposuction intended for clinical use have been commercialized but few papers have compared these procedures in terms of stromal vascular fraction (SVF) or adipose mesenchymal stromal cells (ASC). The objective of the present study was to compare and qualify for clinical use the ASC obtained from fat isolated with the manual or the Bodyjet(®) water-jet-assisted procedure. Although the initial number of cells obtained after collagenase digestion was higher with the manual procedure, the percentage of dead cells, the number of colony forming unit-fibroblast and the phenotype of cells were identical in the SVF at isolation (day 0) and in the ASC populations at day 14. We also showed that the osteogenic and adipogenic differentiation potentials of ASCs were identical between preparations while a slight but significant higher in vitro immunosuppressive effect was observed with ASCs isolated from fat removed with a cannula. The difference in the immunomodulatory effect between ASC populations was, however, not observed in vivo using the delayed-type hypersensitivity (DTH) model. Our data, therefore, indicate that the procedure for fat liposuction does not impact the characteristics or the therapeutic function of ASCs.

Keywords: DTH model; adipose tissue; immunosuppression; liposuction; mesenchymal stem cells.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Characteristics of the stromal vascular fraction at isolation. (A) Representative photographs of fat after water-jet-assisted (Bodyjet) or manual (cannula) lipoaspiration (upper and lower panels, respectively). (B) Average number of mononuclear cells (MNC) obtained after collagenase digestion of the fat tissue removed by the two procedures. (C) Percentage of dead cells in the stromal vascular fraction (SVF) as evaluated as annexinV+ and 7AAD+ cells. (D) Average number of colony forming unit-fibroblast (CFU-F) in the MNC fraction of the SVF. (E) Immunophenotype of the SVF analyzed by flow cytometry, as expressed as percentage of viable cells positive for the indicated markers. n = 7–8 biological replicates; ***p < 0.001.
Figure 2
Figure 2
Characteristics of ASCs after 14 days of expansion. (A) Representative photographs of ASCs isolated from fat obtained after water-jet-assisted (Bodyjet; B) or manual (cannula; C) lipoaspiration (left and right panels, respectively). (B) Cumulative number of ASCs obtained from fat removed by the two procedures during 14 days culture. (C) Average doubling time during the first (days 0–7) or second (days 7–14) week of culture. (D) Average number of colony forming unit-fibroblast (CFU-F) in the ASC populations. (E) Percentage of dead cells in the ASC populations as evaluated as annexinV+ and 7AAD+ cells. (F) Immunophenotype of the ASC populations analyzed by flow cytometry, as expressed as percentage of viable cells positive for the indicated markers. n = 7–8 biological replicates; **: p < 0.01.
Figure 3
Figure 3
Differentiation potential of ASCs. (A) Representative photographs of ASCs isolated from fat obtained after water-jet-assisted (Bodyjet) or manual (cannula) liposuction. Cells were cultured in expansion medium (Ctrl) or adipogenesis inductive conditions (Diff) and stained with Oil Red O. (B) Quantification of Oil Red O dye after extraction from cultures in (A) and spectrometric analysis at the optical density (OD) of 540 nm. (C) Representative photographs of ASCs isolated from fat obtained by two techniques. Cells were cultured in expansion medium (Ctrl) or osteogenesis inductive conditions (Diff) and stained with Alizarin Red S. (D) Quantification of Alizarin Red S dye after extraction from cultures in (C) and spectrometric analysis at the optical density (OD) of 562 nm. n = 8 biological replicates; ***p < 0.001.
Figure 4
Figure 4
In vitro and in vivo immunosuppressive potential of ASCs. (A) Immunosuppressive potential of ASCs isolated from fat obtained after water-jet-assisted (Bodyjet) or manual (cannula) liposuction. The proliferation of peripheral blood mononuclear cells (PBMC) cultured alone (Ctrl) or with ASCs at two ratio (1:10 and 1:5; ASC:PBMC) was quantified. n = 8 biological replicates. (B) Representative photographs of hind paws of mice immunized with PBS (Ctrl), ovalbumin (OVA) or OVA containing ASC isolated from fat obtained by Bodyjet (ASCb) or cannula (ASCc) lipoaspirations. (C) Average thickness of hind paws of mice immunized in (B). n = 2 biological replicates in tetraplicates; *: p < 0.05; ***: p < 0.001;#: p < 0.05 Bodyjet versus cannula.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Maumus M, Guerit D, Toupet K, Jorgensen C, Noel D. Mesenchymal stem cell-based therapies in regenerative medicine: applications in rheumatology. Stem Cell Res Ther (2011) 2(14):6.10.1186/scrt55 - DOI - PMC - PubMed
    1. Maumus M, Peyrafitte JA, D’Angelo R, Fournier-Wirth C, Bouloumie A, Casteilla L, et al. Native human adipose stromal cells: localization, morphology and phenotype. Int J Obes (Lond) (2011) 35(9):1141–53.10.1038/ijo.2010.269 - DOI - PMC - PubMed
    1. Noel D, Caton D, Roche S, Bony C, Lehmann S, Casteilla L, et al. Cell specific differences between human adipose-derived and mesenchymal-stromal cells despite similar differentiation potentials. Exp Cell Res (2008) 314(7):1575–84.10.1016/j.yexcr.2007.12.022 - DOI - PubMed
    1. Schreml S, Babilas P, Fruth S, Orso E, Schmitz G, Mueller MB, et al. Harvesting human adipose tissue-derived adult stem cells: resection versus liposuction. Cytotherapy (2009) 11(7):947–57.10.3109/14653240903204322 - DOI - PubMed
    1. Coleman WP, III. Powered liposuction. Dermatol Surg (2000) 26(4):315–8.10.1046/j.1524-4725.2000.99240.x - DOI - PubMed
-