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. 2016 Jun;22(6):1071-4.
doi: 10.3201/eid2206.160007. Epub 2016 Jun 15.

Experimental Infection and Response to Rechallenge of Alpacas with Middle East Respiratory Syndrome Coronavirus

Gary Crameri, Peter A Durr, Reuben Klein, Adam Foord, Meng Yu, Sarah Riddell, Jessica Haining, Dayna Johnson, Maged G Hemida, Jennifer Barr, Malik Peiris, Deborah Middleton, Lin-Fa Wang

Experimental Infection and Response to Rechallenge of Alpacas with Middle East Respiratory Syndrome Coronavirus

Gary Crameri et al. Emerg Infect Dis. 2016 Jun.

Abstract

We conducted a challenge/rechallenge trial in which 3 alpacas were infected with Middle East respiratory syndrome coronavirus. The alpacas shed virus at challenge but were refractory to further shedding at rechallenge on day 21. The trial indicates that alpacas may be suitable models for infection and shedding dynamics of this virus.

Keywords: MERS-CoV; Middle East respiratory syndrome; SARS; alpaca; camel; coronavirus; experimental animal model; infection; rechallenge; severe acute respiratory syndrome; shedding; vaccine; viruses; zoonoses.

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Figures

Figure
Figure
Virus shedding of MERS-CoV from 3 infected alpacas as detected from the deep nasal swab samples by day after initial infection and reinfection. Viral load was estimated from real-time cycle threshold values and a calibration experiment. Arrow indicates day 21, when the animals were reinfected with MERS-CoV. MERS-COV, Middle East respiratory syndrome coronavirus; TCID, tissue culture infective dose.
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References

    1. Zaki AM, van Boheemen S, Bestebroer TM, Osterhaus AD, Fouchier RA. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med. 2012;367:1814–20. 10.1056/NEJMoa1211721 - DOI - PubMed
    1. Drosten C, Seilmaier M, Corman VM, Hartmann W, Scheible G, Sack S, et al. Clinical features and virological analysis of a case of Middle East respiratory syndrome coronavirus infection. Lancet Infect Dis. 2013;13:745–51. 10.1016/S1473-3099(13)70154-3 - DOI - PMC - PubMed
    1. Reusken CB, Haagmans BL, Müller MA, Gutierrez C, Godeke GJ, Meyer B, et al. Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study. Lancet Infect Dis. 2013;13:859–66. 10.1016/S1473-3099(13)70164-6 - DOI - PMC - PubMed
    1. Haagmans BL, Al Dhahiry SH, Reusken CB, Raj VS, Galiano M, Myers R, et al. Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation. Lancet Infect Dis. 2014;14:140–5. 10.1016/S1473-3099(13)70690-X - DOI - PMC - PubMed
    1. Adney DR, van Doremalen N, Brown VR, Bushmaker T, Scott D, de Wit E, et al. Replication and shedding of MERS-CoV in upper respiratory tract of inoculated dromedary camels. Emerg Infect Dis. 2014;20:1999–2005. 10.3201/eid2012.141280 - DOI - PMC - PubMed

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