Proteomic analysis of stipe explants reveals differentially expressed proteins involved in early direct somatic embryogenesis of the tree fern Cyathea delgadii Sternb
- PMID: 28330564
- DOI: 10.1016/j.plantsci.2017.01.017
Proteomic analysis of stipe explants reveals differentially expressed proteins involved in early direct somatic embryogenesis of the tree fern Cyathea delgadii Sternb
Abstract
Using cyto-morphological analysis of somatic embryogenesis (SE) in the tree fern Cyathea delgadii as a guide, we performed a comparative proteomic analysis in stipe explants undergoing direct SE. Plant material was cultured on hormone-free medium supplemented with 2% sucrose. Phenol extracted proteins were separated using two-dimensional gel electrophoresis (2-DE) and mass spectrometry was performed for protein identification. A total number of 114 differentially regulated proteins was identified during early SE, i.e. when the first cell divisions started and several-cell pro-embryos were formed. Proteins were assigned to seven functional categories: carbohydrate metabolism, protein metabolism, cell organization, defense and stress responses, amino acid metabolism, purine metabolism, and fatty acid metabolism. Carbohydrate and protein metabolism were found to be the most sensitive SE functions with the greatest number of alterations in the intensity of spots in gel. Differences, especially in non-enzymatic and structural protein abundance, are indicative for cell organization, including cytoskeleton rearrangement and changes in cell wall components. The highest induced changes concern those enzymes related to fatty acid metabolism. Global analysis of the proteome reveals several proteins that can represent markers for the first 16days of SE induction and expression in fern. The findings of this research improve the understanding of molecular processes involved in direct SE in C. delgadii.
Keywords: cryptogamic plants; hormone-free medium; mass spectrometry; transmission electron microscopy; two dimensional electrophoresis.
Copyright © 2017 Elsevier B.V. All rights reserved.
Similar articles
-
The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites.Cells. 2021 Jun 4;10(6):1388. doi: 10.3390/cells10061388. Cells. 2021. PMID: 34199921 Free PMC article.
-
Symplasmic Isolation Contributes to Somatic Embryo Induction and Development in the Tree Fern Cyathea delgadii Sternb.Plant Cell Physiol. 2020 Jul 1;61(7):1273-1284. doi: 10.1093/pcp/pcaa058. Plant Cell Physiol. 2020. PMID: 32374847 Free PMC article.
-
Insights from Proteomic Studies into Plant Somatic Embryogenesis.Proteomics. 2018 Mar;18(5-6):e1700265. doi: 10.1002/pmic.201700265. Epub 2018 Feb 23. Proteomics. 2018. PMID: 29369500 Review.
-
Somatic Embryogenesis in Broad-Leaf Woody Plants: What We Can Learn from Proteomics.Methods Mol Biol. 2016;1359:117-29. doi: 10.1007/978-1-4939-3061-6_6. Methods Mol Biol. 2016. PMID: 26619861 Review.
-
Somatic embryogenesis in ferns: a new experimental system.Plant Cell Rep. 2015 May;34(5):783-94. doi: 10.1007/s00299-015-1741-9. Epub 2015 Jan 20. Plant Cell Rep. 2015. PMID: 25599853 Free PMC article.
Cited by
-
Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns.Int J Mol Sci. 2023 Aug 4;24(15):12429. doi: 10.3390/ijms241512429. Int J Mol Sci. 2023. PMID: 37569809 Free PMC article.
-
The Shared Proteome of the Apomictic Fern Dryopteris affinis ssp. affinis and Its Sexual Relative Dryopteris oreades.Int J Mol Sci. 2022 Nov 14;23(22):14027. doi: 10.3390/ijms232214027. Int J Mol Sci. 2022. PMID: 36430514 Free PMC article.
-
The source, level, and balance of nitrogen during the somatic embryogenesis process drive cellular differentiation.Planta. 2022 Nov 11;256(6):113. doi: 10.1007/s00425-022-04009-8. Planta. 2022. PMID: 36367589 Review.
-
Proteomic Analysis of S-Nitrosation Sites During Somatic Embryogenesis in Brazilian Pine, Araucaria angustifolia (Bertol.) Kuntze.Front Plant Sci. 2022 Jun 30;13:902068. doi: 10.3389/fpls.2022.902068. eCollection 2022. Front Plant Sci. 2022. PMID: 35845673 Free PMC article.
-
Biotechnology of the Tree Fern Cyathea smithii (J.D. Hooker; Soft Tree Fern, Katote) II Cell Suspension Culture: Focusing on Structure and Physiology in the Presence of 2,4-D and BAP.Cells. 2022 Apr 20;11(9):1396. doi: 10.3390/cells11091396. Cells. 2022. PMID: 35563701 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources