Mapping the Mouse Cell Atlas by Microwell-Seq
- PMID: 29474909
- DOI: 10.1016/j.cell.2018.02.001
Mapping the Mouse Cell Atlas by Microwell-Seq
Erratum in
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Mapping the Mouse Cell Atlas by Microwell-Seq.Cell. 2018 May 17;173(5):1307. doi: 10.1016/j.cell.2018.05.012. Cell. 2018. PMID: 29775597 No abstract available.
Abstract
Single-cell RNA sequencing (scRNA-seq) technologies are poised to reshape the current cell-type classification system. However, a transcriptome-based single-cell atlas has not been achieved for complex mammalian systems. Here, we developed Microwell-seq, a high-throughput and low-cost scRNA-seq platform using simple, inexpensive devices. Using Microwell-seq, we analyzed more than 400,000 single cells covering all of the major mouse organs and constructed a basic scheme for a mouse cell atlas (MCA). We reveal a single-cell hierarchy for many tissues that have not been well characterized previously. We built a web-based "single-cell MCA analysis" pipeline that accurately defines cell types based on single-cell digital expression. Our study demonstrates the wide applicability of the Microwell-seq technology and MCA resource.
Keywords: Microwell-seq; cell type classification; cellular heterogeneity; cross-tissue cellular network; mammalian cell map; mouse cell atlas; scMCA analysis; single cell RNA-seq; single-cell analysis; transcriptome analysis.
Copyright © 2018 Elsevier Inc. All rights reserved.
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