Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018:1764:59-71.
doi: 10.1007/978-1-4939-7759-8_4.

Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip

Sandip Kaledhonkar  1 , Ziao Fu  2 , Howard White  3 , Joachim Frank  4   5
Affiliations

Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip

Sandip Kaledhonkar et al. Methods Mol Biol. 2018.

Abstract

With the advent of direct electron detectors, cryo-EM has become a popular choice for molecular structure determination. Among its advantages over X-ray crystallography are (1) no need for crystals, (2) much smaller sample volumes, and (3) the ability to determine multiple structures or conformations coexisting in one sample. In principle, kinetic experiments can be done using standard cryo-EM, but mixing and freezing grids require several seconds. However, many biological processes are much faster than that time scale, and the ensuing short-lived states of the molecules cannot be captured. Here, we lay out a detailed protocol for how to capture such intermediate states on the millisecond time scale with time-resolved cryo-EM.

Keywords: Microfluidic chip; Short-lived intermediates heterogeneity; Single-particle cryo-EM; Spraying; Time-resolved.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

-