Validating Resolution Revolution
- PMID: 29606592
- DOI: 10.1016/j.str.2018.03.004
Validating Resolution Revolution
Erratum in
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Validating Resolution Revolution.Structure. 2018 Dec 4;26(12):1678. doi: 10.1016/j.str.2018.10.028. Structure. 2018. PMID: 30517886 No abstract available.
Abstract
Recent advances in instrumentation and image-processing software have resulted in a resolution revolution in cryo-electron microscopy (cryo-EM) and a surge in the popularity of this technique. However, despite technical progress and hundreds of structures determined so far, development of standards assessing the agreement between the cryo-EM map and the respective model has fallen behind. Here we establish a validation procedure evaluating this agreement and applied it to a set of 565 cryo-EM structures. Analysis of the results revealed that three-quarters of the validated structures exhibit moderate or low agreement between the map and the corresponding model, mostly due to limited structural features possessed by these maps. Model re-refinement significantly improved the agreement for only one-fifth of the structures, reaffirming the necessity to re-evaluate map resolution. The presented procedure provides an approach to re-estimate the resolution of cryo-EM map areas interpreted by the model.
Keywords: FSC; FSC-based validation; RSCC-based validation; cryo-EM; estimation of resolution; quality criteria; validation.
Copyright © 2018 Elsevier Ltd. All rights reserved.
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