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. 1988 Dec;85(24):9557-61.
doi: 10.1073/pnas.85.24.9557.

Polarized apical distribution of glycosyl-phosphatidylinositol-anchored proteins in a renal epithelial cell line

M P Lisanti  1 , M Sargiacomo, L Graeve, A R Saltiel, E Rodriguez-Boulan
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Polarized apical distribution of glycosyl-phosphatidylinositol-anchored proteins in a renal epithelial cell line

M P Lisanti et al. Proc Natl Acad Sci U S A. 1988 Dec.

Abstract

Polarized epithelial cell monolayers contain two distinct plasma membrane domains as delineated by the presence of tight junctions--i.e., an apical surface that faces the external environment and a basolateral surface that functions both in cell-cell contact and cell-substrate attachment. Central to the understanding of epithelial cell polarity is the question of how such cell-surface specializations are generated. A different class of membrane glycoproteins has recently emerged that may yield new insight into the mechanism underlying the biogenesis of this polarity. Members of this class contain a large extracellular protein domain linked to the membrane via glycosyl-phosphatidylinositol. Using a polarized renal epithelial cell line (Madin-Darby canine kidney), we identified endogenous glycosyl-phosphatidylinositol-anchored proteins through release by a phosphatidylinositol-specific phospholipase C. Six glycosyl-phosphatidylinositol-anchored proteins of 110, 85, 70, 55, 38, and 35 kDa were identified and appeared to be restricted to the apical surface. Our data are consistent with the notion that the glycosyl-phosphatidylinositol membrane anchor may contain the necessary information for "targeting" to the apical surface.

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References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Cell Biol. 1963 May;17:375-412 - PubMed
    1. Anal Biochem. 1976 Jan;70(1):241-50 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8 - PubMed
    1. FEBS Lett. 1977 Oct 1;82(1):143-6 - PubMed

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