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. 1985 Dec;82(24):8340-4.
doi: 10.1073/pnas.82.24.8340.

Human apolipoprotein B-100: cloning, analysis of liver mRNA, and assignment of the gene to chromosome 2

S W Law, K J Lackner, A V Hospattankar, J M Anchors, A Y Sakaguchi, S L Naylor, H B Brewer Jr

Human apolipoprotein B-100: cloning, analysis of liver mRNA, and assignment of the gene to chromosome 2

S W Law et al. Proc Natl Acad Sci U S A. 1985 Dec.

Abstract

Human apolipoprotein B-100 (apo B-100) is the major apolipoprotein of low density lipoproteins and the principal ligand for interaction with the low density lipoprotein receptor. The human apo B-100 gene has been inserted into a lambda gt-11 expression vector, and the apo B-100 cDNA clones have been identified by screening with a monospecific apo B-100 antiserum, by screening with synthetic oligonucleotides based on the amino acid sequence of peptides isolated from apo B-100, and by immunoblot analysis of the expressed protein with a monoclonal antibody to apo B-100. The complete nucleotide and derived-amino acid sequence of a 1.7-kilobase cDNA clone of apo B-100 was determined. The 560-amino acid residues of apo B-100 contain no unique linear or repeating sequences of amino acids. The computer-predicted conformation of the apo B-100 protein contains segments of helical structure; however, a large portion of the protein is organized into beta-structure. The beta-structure may be important in lipid-apo B-100 interactions in low density lipoprotein and may contribute to the insolubility of delipidated apo B-100 in aqueous buffers. RNA blot hybridization analysis of liver mRNA utilizing a Nco I/HindIII apo B-100 cDNA probe revealed that the apo B-100 mRNA is 15-18 kilobases long, which is of sufficient size to code for a 250-387 kDa apolipoprotein, the proposed molecular size of delipidated plasma apo B-100. The gene for human apo B-100 has been localized to chromosome 2 by filter hybridization of human-mouse somatic cell hybrids utilizing a 400-base-pair Nco I/HindIII apo B-100 cDNA probe. This location is in contrast to the low density lipoprotein receptor that has been localized to chromosome 19. The cloning of human apo B-100 has provided new insights into the structure and physicochemical properties of apo B-100 and will facilitate studies on the factors modulating apo B-100 biosynthesis and the expression of the apo B-100 gene in patients with dyslipoproteinemias.

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References

    1. Lancet. 1971 Oct 30;2(7731):971-2 - PubMed
    1. Biochemistry. 1985 Mar 12;24(6):1569-78 - PubMed
    1. Mol Cell Biochem. 1975 Jan 31;6(1):43-50 - PubMed
    1. Physiol Rev. 1976 Apr;56(2):259-316 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 - PubMed

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