Laser-Induced Fluorometry for Capillary Electrophoresis
- PMID: 30079538
- DOI: 10.1002/tcr.201800051
Laser-Induced Fluorometry for Capillary Electrophoresis
Abstract
Laser-induced fluorometry (LIF) has achieved the detection of single molecules, which ranks it among the most sensitive of detection techniques, whereas capillary electrophoresis (CE) is known as a powerful separation method with resolution that is beyond the reach of many other types of chromatography. Therefore, a coupling of LIF with CE has established an unrivaled analytical technique in terms of sensitivity and resolution. CE-LIF has demonstrated excellent performance in bioanalytical chemistry for the high-resolution separation and highly sensitive detection of DNAs, proteins, and small bioactive molecules. This review describes the CE-LIF methods developed by the author's group that include indirect and direct detection using diode lasers, post-column derivatization, and Hadamard transformation, as well as applications to the binding assays of specific DNA immunoassays of proteins and to the determination of anticancer drugs.
Keywords: DNA sequencing; capillary electrophoresis; laser-induced fluorescence; micellar electrokinetic chromatography.
© 2019 The Chemical Society of Japan & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Similar articles
-
LIF detection of peptides and proteins in CE.Electrophoresis. 2007 Jan;28(1-2):208-32. doi: 10.1002/elps.200500790. Electrophoresis. 2007. PMID: 17136735 Review.
-
Recent developments and applications of capillary electrophoresis with laser-induced fluorescence detection in biological samples.J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jun 15;929:180-6. doi: 10.1016/j.jchromb.2013.04.028. Epub 2013 Apr 26. J Chromatogr B Analyt Technol Biomed Life Sci. 2013. PMID: 23685428 Review.
-
Critical review of recent developments in fluorescence detection for capillary electrophoresis.Electrophoresis. 1997 Nov;18(12-13):2279-90. doi: 10.1002/elps.1150181218. Electrophoresis. 1997. PMID: 9456042 Review.
-
Capillary electrophoresis hyphenated with UV-native-laser induced fluorescence detection (CE/UV-native-LIF).Electrophoresis. 2017 Jan;38(1):135-149. doi: 10.1002/elps.201600248. Epub 2016 Sep 5. Electrophoresis. 2017. PMID: 27445082 Review.
-
Fast detection of a (CA)18 microsatellite repeat in the IgE receptor gene by capillary electrophoresis with laser-induced fluorescence detection.Electrophoresis. 1998 Feb;19(2):249-55. doi: 10.1002/elps.1150190218. Electrophoresis. 1998. PMID: 9548287
Cited by
-
A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy.Biomedicines. 2020 Jul 30;8(8):255. doi: 10.3390/biomedicines8080255. Biomedicines. 2020. PMID: 32751506 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources