Bacterial Outer Membrane Vesicles (OMVs)-based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV
- PMID: 31141982
- PMCID: PMC6631769
- DOI: 10.3390/vaccines7020046
Bacterial Outer Membrane Vesicles (OMVs)-based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV
Abstract
Vaccination is the most functional medical intervention to prophylactically control severe diseases caused by human-to-human or animal-to-human transmissible viral pathogens. Annually, seasonal influenza epidemics attack human populations leading to 290-650 thousand deaths/year worldwide. Recently, a novel Middle East Respiratory Syndrome Coronavirus emerged. Together, those two viruses present a significant public health burden in areas where they circulate. Herein, we generated a bacterial outer membrane vesicles (OMVs)-based vaccine presenting the antigenic stable chimeric fusion protein of the H1-type haemagglutinin (HA) of the pandemic influenza A virus (H1N1) strain from 2009 (H1N1pdm09) and the receptor binding domain (RBD) of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (OMVs-H1/RBD). Our results showed that the chimeric antigen could induce specific neutralizing antibodies against both strains leading to protection of immunized mice against H1N1pdm09 and efficient neutralization of MERS-CoV. This study demonstrate that OMVs-based vaccines presenting viral antigens provide a safe and reliable approach to protect against two different viral infections.
Keywords: H1N1pdm; MERS-CoV; OMVs; influenza vaccine.
Conflict of interest statement
The authors declare no conflict of interest.
Figures
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- HHSN272201400006C/National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services/International
- 5175/Science and Technology Development Fund (STDF) in Egypt/International
- T. C1/DFG-funded Collaborative Research Centres 1021/International
- TP A4/Transregional Collaborative Research Centre TRR 84/International
- TTU 01.803/German Centre for Infection Research/International
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