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. 2019 Sep 25:7:e7739.
doi: 10.7717/peerj.7739. eCollection 2019.

Identification of genes encoding ALMT and MATE transporters as candidate aluminum tolerance genes from a typical acid soil plant, Psychotria rubra (Rubiaceae)

Akira Iguchi  1 , Kazutsuka Sanmiya  2 , Kenta Watanabe  3
Affiliations

Identification of genes encoding ALMT and MATE transporters as candidate aluminum tolerance genes from a typical acid soil plant, Psychotria rubra (Rubiaceae)

Akira Iguchi et al. PeerJ. .

Abstract

To understand how tropical plants have adapted to acid soils, we analyzed the transcriptome of seedlings of Psychotria rubra, a typical species found on acid soils. Using RNA-seq, we identified 22,798 genes, including several encoding proteins of the Al3+-activated malate transporter (ALMT) and multidrug and toxic compound extrusion (MATE) families. Molecular phylogenetic analysis of ALMTs and MATEs revealed the grouping of those from P. rubra, which may be useful to select targets for elucidating the molecular basis of P. rubra adaptation to acid soils in the future. The transcriptome datasets obtained in this study would help us to further understand the physiological and ecological aspects of soil adaptation of Psychotria species.

Keywords: ALMT; Acid soils; MATE; Plant; Psychotria; Transcriptome.

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Conflict of interest statement

The authors declare there are no competing interests.

Figures

Figure 1
Figure 1. Psychotria rubra.
(A) Shrub on Mount Nago-dake, Okinawa, Japan. (B) Seedlings.
Figure 2
Figure 2. Phylogenetic tree of ALMTs of P. rubra and related proteins.
Sequences of P. rubra are shown using red shades. For sequences other than P. rubra ALMTs, the labels show the UniProt database ID and ALMT type, and ones from Dreyer et al. (2012). Number at each node is the bootstrap value.
Figure 3
Figure 3. Phylogenetic tree of MATEs of P. rubra and related proteins.
Sequences of P. rubra are shown using red shades. For sequences other than P. rubra MATEs, the labels are UniProt database IDs, and ones from Liu et al. (2016). Number at each node is the bootstrap value.
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Grants and funding

This study was supported by a grant from the Nissei Science Foundation to Akira Iguchi (No. 03) and JSPS KAKENHI Grants to Kenta Watanabe (Nos. JP26840130 and JP18K14782). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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