Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Nov;39(11):6259-6263.
doi: 10.21873/anticanres.13835.

Expression of Kita-Kyushu Lung Cancer Antigen-1 as Detected by a Novel Monoclonal Antibody in Gastric Cancer

Yoshihito Takahashi  1   2 , Takashi Fukuyama  3 , Nobue Futawatari  4 , Yoshinobu Ichiki  5 , Haruki Ohmiya  6 , Motohiro Chuman  1   7 , Tetsuro Yamashita  6 , Naoki Hiki  7 , Yusuke Kumamoto  2 , Hitoshi Yamazaki  8 , Hiroshi Nonoguchi  9 , Yatsushi Nishi  1 , Noritada Kobayashi  10
Affiliations

Expression of Kita-Kyushu Lung Cancer Antigen-1 as Detected by a Novel Monoclonal Antibody in Gastric Cancer

Yoshihito Takahashi et al. Anticancer Res. 2019 Nov.

Abstract

Background/aim: Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a known cancer/testis antigen. Our group has previously shown KK-LC-1 gene expression in gastric cancer. However, could not be detected the KK-LC-1 protein due to the lack of an appropriate antibody. Here, we assessed our original monoclonal antibody (Kmab34B3) and, using it, assessed the expression of KK-LC-1 in gastric cancer.

Patients and methods: We evaluated an original monoclonal antibody against KK-LC-1 (Kmab34B3), and used this antibody to compare KK-LC-1 protein expression in tumour and non-tumour stomach cells from gastric cancer patients.

Results: Kmab34B3 stained testicular germ cells, and tumour cells in nine out of 11 (82%) specimens. In non-tumorous areas, Kmab34B3 stained 13 out of 29 (45%) pyloric gland specimens. Furthermore, Kmab34B3 also stained intestinal metaplasia positive and negative areas.

Conclusion: Kmab34B3 was able to detect KK-LC-1 protein within tumour cells and the pyloric gland where the gene has been shown to be expressed. Therefore, it might be an attractive tool for detecting KK-LC-1 expression in precancerous and cancerous stomach cells.

Keywords: Gastric cancer; Kita-Kyushu lung cancer antigen-1.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

LinkOut - more resources

-