Ultra-Rapid Real-Time RT-PCR Method for Detecting Middle East Respiratory Syndrome Coronavirus Using a Mobile PCR Device, PCR1100
- PMID: 31875608
- DOI: 10.7883/yoken.JJID.2019.400
Ultra-Rapid Real-Time RT-PCR Method for Detecting Middle East Respiratory Syndrome Coronavirus Using a Mobile PCR Device, PCR1100
Abstract
Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) is usually diagnosed through highly sensitive and specific genetic tests such as real-time reverse transcription polymerase chain reaction (RT-PCR). Currently, two real-time RT-PCR assays targeting the upE and ORF1a regions of the MERS-CoV genome are widely used, and these are the standard assays recommended by the World Health Organization (WHO). The MERS outbreaks to date suggest that rapid diagnosis and subsequent isolation of infected patients, particularly superspreaders, are critical for containment. However, conventional real-time RT-PCR assays require large laboratory instruments, and amplification takes approximately 2 h. These disadvantages limit rapid diagnosis. Here, an ultra-rapid real-time RT-PCR test was established comprising a multiplex assay for upE and ORF1a running on a mobile PCR1100 device. As few as five copies of the MERS-CoV RNA can be detected within 20 min using the standard WHO assays in the mobile PCR device, with the sensitivity and specificity being similar to those of a conventional real-time PCR instrument such as the LightCyler, thereby enabling timely intervention to control MERS-CoV infection.
Keywords: MERS coronavirus (MERS-CoV); Middle East Respiratory Syndrome (MERS); PCR1100; ultra-rapid real-time RT-PCR.
Similar articles
-
Performance Evaluation of the PowerChek MERS (upE & ORF1a) Real-Time PCR Kit for the Detection of Middle East Respiratory Syndrome Coronavirus RNA.Ann Lab Med. 2017 Nov;37(6):494-498. doi: 10.3343/alm.2017.37.6.494. Ann Lab Med. 2017. PMID: 28840986 Free PMC article.
-
Evaluation and Clinical Validation of Two Field-Deployable Reverse Transcription-Insulated Isothermal PCR Assays for the Detection of the Middle East Respiratory Syndrome-Coronavirus.J Mol Diagn. 2017 Nov;19(6):817-827. doi: 10.1016/j.jmoldx.2017.06.007. Epub 2017 Aug 12. J Mol Diagn. 2017. PMID: 28807812 Free PMC article.
-
MERS-CoV diagnosis: An update.J Infect Public Health. 2016 May-Jun;9(3):216-9. doi: 10.1016/j.jiph.2016.04.005. Epub 2016 Apr 20. J Infect Public Health. 2016. PMID: 27106390 Free PMC article. Review.
-
Korean Society for Laboratory Medicine Practice Guidelines for the Molecular Diagnosis of Middle East Respiratory Syndrome During an Outbreak in Korea in 2015.Ann Lab Med. 2016 May;36(3):203-8. doi: 10.3343/alm.2016.36.3.203. Ann Lab Med. 2016. PMID: 26915607 Free PMC article. Review.
-
Performance and clinical validation of the RealStar MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA.J Clin Virol. 2014 Jun;60(2):168-71. doi: 10.1016/j.jcv.2014.03.012. Epub 2014 Mar 28. J Clin Virol. 2014. PMID: 24726679 Free PMC article.
Cited by
-
Dry loop-mediated isothermal amplification assay for detection of SARS-CoV-2 from clinical specimens.Fujita Med J. 2023 May;9(2):84-89. doi: 10.20407/fmj.2022-003. Epub 2022 Jul 22. Fujita Med J. 2023. PMID: 37234399 Free PMC article.
-
Evaluation of a real-time mobile PCR device (PCR 1100) for the detection of the rabies gene in field samples.Trop Med Health. 2023 Mar 17;51(1):17. doi: 10.1186/s41182-023-00501-3. Trop Med Health. 2023. PMID: 36932428 Free PMC article.
-
Performance Evaluation of a Novel Ultrafast Molecular Diagnostic Device Integrated With Microfluidic Chips and Dual Temperature Modules.Front Bioeng Biotechnol. 2022 May 19;10:895236. doi: 10.3389/fbioe.2022.895236. eCollection 2022. Front Bioeng Biotechnol. 2022. PMID: 35662850 Free PMC article.
-
A multi-method analytical approach to predicting young adults' intention to invest in mHealth during the COVID-19 pandemic.Telemat Inform. 2022 Mar;68:101765. doi: 10.1016/j.tele.2021.101765. Epub 2021 Dec 22. Telemat Inform. 2022. PMID: 34955594 Free PMC article.
-
Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification Assay.Pathogens. 2021 Sep 25;10(10):1237. doi: 10.3390/pathogens10101237. Pathogens. 2021. PMID: 34684186 Free PMC article.
MeSH terms
LinkOut - more resources
Full Text Sources
Research Materials