Assessment of different selective agar media for enumeration and isolation of Listeria from dairy products
- PMID: 3235719
- DOI: 10.1017/s0022029900033367
Assessment of different selective agar media for enumeration and isolation of Listeria from dairy products
Abstract
Different selective agar media were compared for the recovery and isolation of five species of Listeria from raw milk and cheese. The selective media examined were Beerens medium, MacBride medium and that described by Dominguez et al. (1984) with 6 mg/l acriflavine, listeria selective agar medium (LSAM), and LSAM with 12 mg/l acriflavine (LSAM X 2A); a non-selective yeast glucose Lemco agar was included for comparison. When the difference between listeria and the natural microflora of raw milk and cheese was 10(2) cfu/ml, listeria could be isolated by direct plating on all media tested. When it was lower than 10(3)-10(4) cfu/ml, listeria were isolated by direct plating only on LSAM and LSAM X 2A. When the difference was greater than 10(4) cfu/ml, a previous enrichment was necessary to isolate them. LSAM and LSAM X 2A media performed better than the other media tested for isolating listeria by direct plating and improved their isolation from dairy products. This superior performance was evaluated by the ability of these media to support colony formation of different species of Listeria tested, the easy recognition of these colonies from those formed by other microorganisms and by their capacity to inhibit the natural microflora of these foods.
Similar articles
-
Efficacy of direct plating media for recovering Listeria monocytogenes from foods.Int J Food Microbiol. 1990 Mar;10(2):143-55. doi: 10.1016/0168-1605(90)90062-a. Int J Food Microbiol. 1990. PMID: 2119210
-
Evaluation of enrichment procedures for recovering Listeria monocytogenes from dairy products.Int J Food Microbiol. 1989 Nov;9(3):249-68. doi: 10.1016/0168-1605(89)90094-9. Int J Food Microbiol. 1989. PMID: 2518230
-
Direct plating technique for enumeration of Listeria monocytogenes in foods.J Assoc Off Anal Chem. 1988 May-Jun;71(3):647-50. J Assoc Off Anal Chem. 1988. PMID: 3134335
-
New chromogenic plating media for detection and enumeration of pathogenic Listeria spp.--an overview.Int J Food Microbiol. 2004 Aug 15;95(1):1-9. doi: 10.1016/j.ijfoodmicro.2004.01.025. Int J Food Microbiol. 2004. PMID: 15240069 Review.
-
Bacterial culture through selective and non-selective conditions: the evolution of culture media in clinical microbiology.New Microbes New Infect. 2019 Nov 30;34:100622. doi: 10.1016/j.nmni.2019.100622. eCollection 2020 Mar. New Microbes New Infect. 2019. PMID: 31956419 Free PMC article. Review.
Cited by
-
Highly selective medium for isolation of Listeria monocytogenes from food.Appl Environ Microbiol. 1990 Oct;56(10):3154-7. doi: 10.1128/aem.56.10.3154-3157.1990. Appl Environ Microbiol. 1990. PMID: 2126701 Free PMC article.
-
Listeria monocytogenes, a food-borne pathogen.Microbiol Rev. 1991 Sep;55(3):476-511. doi: 10.1128/mr.55.3.476-511.1991. Microbiol Rev. 1991. PMID: 1943998 Free PMC article. Review.