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. 2020 Mar 26;18(1):297-302.
doi: 10.1007/s40201-020-00467-y. eCollection 2020 Jun.

The effect of Propolis on inhibition of Aspergillus parasiticus growth, aflatoxin production and expression of aflatoxin biosynthesis pathway genes

Hamideh Mahmoodzadeh Hosseini  1 , Siavash Hamzeh Pour  2 , Jafar Amani  1 , Sima Jabbarzadeh  3 , Mostafa Hosseinabadi  4 , Seyed Ali Mirhosseini  1
Affiliations

The effect of Propolis on inhibition of Aspergillus parasiticus growth, aflatoxin production and expression of aflatoxin biosynthesis pathway genes

Hamideh Mahmoodzadeh Hosseini et al. J Environ Health Sci Eng. .

Abstract

Background and purpose: Aflatoxins are one of the most important mycotoxins, which have been classified as Group I carcinogenic compounds by the International Agency for Research on Cancer. This investigation aimed to examine the effect of Propolis on inhibition of the Aspergillus parasiticus growth, aflatoxin production and expression of aflatoxin biosynthesis pathway genes.

Materials and methods: A standard strain of Aspergillus parasiticus (ATCC 15517) was used to perform antifungal susceptibility test, using a microdilution method in accordance with the CLSI M38-A2 guidelines. The aflatoxin concentrations in the control and treated media were determined by HPLC. Also, the quantitative changes in the level of nor-1, ver-1 and omtA genes expression in aflatoxin biosynthetic pathway were analyzed using Real-Time PCR method.

Results: The results showed that the minimum inhibitory concentrations (MIC) of propolis was 100 µg/ml. The results showed that total levels of aflatoxin decreased from 386.1 ppm to 3.01 ppm at 50 µg/ml of propolis. In addition, quantitative real-time PCR analysis showed that the level of nor-1, ver-1 and omtA genes expression was significantly decreased after treatment with propolis extract.

Conclusions: The findings reveal that propolis extract, have a significant inhibitory effect on important genes for aflatoxin biosynthesis pathway in aflatoxin production.

Keywords: Aflatoxin; Aspergillus parasiticus; Gene expression; Propolis.

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Conflict of interest statement

Conflict of interestThere is no conflict of interest.

Figures

Fig. 1
Fig. 1
Comparison of nor-1, ver-1 and omtA genes expression in the presence of propolis extract
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References

    1. Hedayati MT, Omran SM, Soleymani A, Armaki MT. Aflatoxins in food products in Iran: a review of the literature. Jundishapur J Microbiol. 2016;9(7). 10.5812/jjm.33235. - PMC - PubMed
    1. Creppy EE. Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicol Lett. 2002;127(1–3):19–28. doi: 10.1016/S0378-4274(01)00479-9. - DOI - PubMed
    1. Dehghan P, Zeyni F, Rezaei R, Jebali A, Kordbacheh P, Mahmoudi M. Detection of aflr gene and toxigenicity of Aspergillus flavus group isolated from patients with fungal sinusitis. 2008.
    1. Ghanbari R, Molaee Aghaee E, Rezaie S, Jahed Khaniki G, Alimohammadi M, Soleimani M, et al. The inhibitory effect of lactic acid bacteria on aflatoxin production and expression of aflR gene in Aspergillus parasiticus. J Food Saf. 2018;8(1):e12413. doi: 10.1111/jfs.12413. - DOI
    1. Mohseni R, Noorbakhsh F, Moazeni M, Nasrollahi Omran A, Rezaie S. Antitoxin characteristic of licorice extract: the inhibitory effect on aflatoxin production in Aspergillus parasiticus. J Food Saf. 2014;34(2):119–25. doi: 10.1111/jfs.12104. - DOI

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