Disruption of microfilament organization and deregulation of disk membrane morphogenesis by cytochalasin D in rod and cone photoreceptors
- PMID: 3397406
- DOI: 10.1002/cne.902720202
Disruption of microfilament organization and deregulation of disk membrane morphogenesis by cytochalasin D in rod and cone photoreceptors
Abstract
Morphogenesis of photoreceptor outer segment disks appears to occur by an evagination of the ciliary plasma membrane (Steinberg et al., J Comp Neurol 190:501-519, '80). We tested if polymerized actin (F-actin) was necessary for the regulation of this postulated process by incubating Xenopus eyecups with 5 or 25 microM cytochalasin D for 6-28 hours. During the second hour, the incubation medium contained 3H-leucine. Both concentrations of cytochalasin resulted in: 1) dissolution of the rhodamine-phalloidin labeling pattern of photoreceptors, and 2) collapse of the calycal processes (which are normally filled with actin filaments) and disappearance of the inner segment microfilaments. In addition, the few most basal rod and cone outer segment disks appeared several times their normal diameter. These oversized disks had incorporated 3H-leucine and extended along the margin of the outer or inner segment. The nature of the overgrown disks is consistent only with a morphogenetic process involving evaginations of the ciliary plasma membrane. Deregulation by cytochalasin D was manifest by excessive growth of a few nascent disks rather than normal growth of many. Therefore, the normal network of actin filaments is apparently not necessary for continued evagination of the membrane, but it does seem to be an essential part of the mechanism that initiates the evagination of the ciliary plasma membrane and/or the mechanism that controls how far nascent disks grow.
Similar articles
-
The actin network in the ciliary stalk of photoreceptors functions in the generation of new outer segment discs.J Comp Neurol. 1996 Dec 2;376(1):128-42. doi: 10.1002/(SICI)1096-9861(19961202)376:1<128::AID-CNE8>3.0.CO;2-5. J Comp Neurol. 1996. PMID: 8946288
-
Membrane and microfilament organization and vasopressin action in transporting epithelia.Soc Gen Physiol Ser. 1987;42:259-75. Soc Gen Physiol Ser. 1987. PMID: 3333192 Review. No abstract available.
-
Cytochalasin D disrupts outer segment disc morphogenesis in situ in rabbit retina.Invest Ophthalmol Vis Sci. 1989 Feb;30(2):339-42. Invest Ophthalmol Vis Sci. 1989. PMID: 2914762
-
Actin filaments and photoreceptor membrane turnover.Bioessays. 1991 Apr;13(4):171-8. doi: 10.1002/bies.950130405. Bioessays. 1991. PMID: 1859395 Review.
-
Actin-dependent myoid elongation in teleost rod inner/outer segments occurs in the absence of net actin polymerization.Cell Motil Cytoskeleton. 1992;21(3):235-51. doi: 10.1002/cm.970210307. Cell Motil Cytoskeleton. 1992. PMID: 1581976
Cited by
-
Ciliary tip actin dynamics regulate photoreceptor outer segment integrity.Nat Commun. 2024 May 21;15(1):4316. doi: 10.1038/s41467-024-48639-w. Nat Commun. 2024. PMID: 38773095 Free PMC article.
-
NUDC is critical for rod photoreceptor function, maintenance, and survival.FASEB J. 2024 Mar 15;38(5):e23518. doi: 10.1096/fj.202301641RR. FASEB J. 2024. PMID: 38441532
-
Expression of two major isoforms of MYO7A in the retina: Considerations for gene therapy of Usher syndrome type 1B.Vision Res. 2023 Nov;212:108311. doi: 10.1016/j.visres.2023.108311. Epub 2023 Aug 15. Vision Res. 2023. PMID: 37586294 Free PMC article.
-
A Ciliary Branched Actin Network Drives Photoreceptor Disc Morphogenesis.Adv Exp Med Biol. 2023;1415:507-511. doi: 10.1007/978-3-031-27681-1_74. Adv Exp Med Biol. 2023. PMID: 37440079
-
The WAVE complex drives the morphogenesis of the photoreceptor outer segment cilium.Proc Natl Acad Sci U S A. 2023 Mar 21;120(12):e2215011120. doi: 10.1073/pnas.2215011120. Epub 2023 Mar 14. Proc Natl Acad Sci U S A. 2023. PMID: 36917665 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous