Limited Neutralization of Authentic Severe Acute Respiratory Syndrome Coronavirus 2 Variants Carrying E484K In Vitro

doi:10.1093/infdis/jiab355

. 2021 Oct 13;224(7):1109-1114.

doi: 10.1093/infdis/jiab355.

Limited Neutralization of Authentic Severe Acute Respiratory Syndrome Coronavirus 2 Variants Carrying E484K In Vitro

Marek Widera¹, Alexander Wilhelm¹, Sebastian Hoehl¹, Christiane Pallas¹, Niko Kohmer¹, Timo Wolf^{2

3}, Holger F Rabenau¹, Victor M Corman^{4

5}, Christian Drosten^{4

5}, Maria J G T Vehreschild^{2

3}, Udo Goetsch⁶, Rene Gottschalk⁶, Sandra Ciesek^{1

5

7}

Affiliations

¹ Institute for Medical Virology, University Hospital Frankfurt, Goethe University Frankfurt, Frankfurt am Main, Germany.
² Department of Internal Medicine, Infectious Diseases, University Hospital Frankfurt, Goethe University Frankfurt, Frankfurt am Main, Germany.
³ University Center for Infectious Diseases, University Hospital Frankfurt, Goethe University, Frankfurt am Main, Germany.
⁴ Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
⁵ German Center for Infection Research, Braunschweig, Germany.
⁶ Public Health Department of the City of Frankfurt am Main, Frankfurt am Main, Germany.
⁷ Fraunhofer Institute for Molecular Biology and Applied Ecology, Branch Translational Medicine and Pharmacology, Frankfurt am Main, Germany.

PMID: 34223909
PMCID: PMC8344430
DOI: 10.1093/infdis/jiab355

Limited Neutralization of Authentic Severe Acute Respiratory Syndrome Coronavirus 2 Variants Carrying E484K In Vitro

Marek Widera et al. J Infect Dis. 2021.

. 2021 Oct 13;224(7):1109-1114.

doi: 10.1093/infdis/jiab355.

Authors

Affiliations

¹ Institute for Medical Virology, University Hospital Frankfurt, Goethe University Frankfurt, Frankfurt am Main, Germany.
² Department of Internal Medicine, Infectious Diseases, University Hospital Frankfurt, Goethe University Frankfurt, Frankfurt am Main, Germany.
³ University Center for Infectious Diseases, University Hospital Frankfurt, Goethe University, Frankfurt am Main, Germany.
⁴ Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
⁵ German Center for Infection Research, Braunschweig, Germany.
⁶ Public Health Department of the City of Frankfurt am Main, Frankfurt am Main, Germany.
⁷ Fraunhofer Institute for Molecular Biology and Applied Ecology, Branch Translational Medicine and Pharmacology, Frankfurt am Main, Germany.

PMID: 34223909
PMCID: PMC8344430
DOI: 10.1093/infdis/jiab355

Abstract

Whether monoclonal antibodies are able to neutralize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern has been investigated using pseudoviruses. In this study we show that bamlanivimab, casirivimab, and imdevimab efficiently neutralize authentic SARS-CoV-2, including variant B.1.1.7 (alpha), but variants B.1.351 (beta) and P.2 (zeta) were resistant against bamlanivimab and partially resistant to casirivimab. Whether antibodies are able to neutralize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variantshas been investigated using pseudoviruses. We show that authentic SARS-CoV-2 carrying E484K were resistant against bamlanivimab and less susceptible to casirivimab, convalescent and vaccine-elicited sera.

Keywords: SARS-CoV-2; bamlanivimab; casirivimab; convalescent; coronavirus; imdevimab; monoclonal antibodies; vaccination.

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Limited Neutralization of Authentic Severe Acute Respiratory Syndrome Coronavirus 2 Variants Carrying E484K In Vitro

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Limited Neutralization of Authentic Severe Acute Respiratory Syndrome Coronavirus 2 Variants Carrying E484K In Vitro

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