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. 2022 Sep 13;14(18):3770.
doi: 10.3390/nu14183770.

Association between Single Nucleotide Polymorphisms of SULT1A1, SULT1C4, ABCC2 and Phase II Flavanone Metabolites Excretion after Orange Juice Intake

Layanne Nascimento Fraga  1 , Dragan Milenkovic  2 , Franco Maria Lajolo  1 , Neuza Mariko Aymoto Hassimotto  1
Affiliations

Association between Single Nucleotide Polymorphisms of SULT1A1, SULT1C4, ABCC2 and Phase II Flavanone Metabolites Excretion after Orange Juice Intake

Layanne Nascimento Fraga et al. Nutrients. .

Abstract

Citrus fruits and juices are a major source of dietary flavanones, and the regular consumption of these foods is inversely associated with the development of cardiometabolic diseases. However, the biological benefits depend on the bioavailability of these compounds, and previous studies have reported a large interindividual variability in the absorption and excretion of these compounds. Different factors, such as age, gender or genetic polymorphism of genes coding enzymes involved in the metabolism and transport of the flavanones, may explain this heterogeneity. This study aimed to assess the impact of single nucleotide polymorphism of sulfotransferases SULT1A1 and SULT1C4, and ABCC2 transporter genes on excretion of phase II flavanone metabolites in volunteers after 24 h of orange juice intake. Forty-six volunteers ingested a single dose of 500 mL of orange juice and 24-h urine was collected. The hesperetin and naringenin phase II metabolites were quantified in urine, and SNPs in SULT1A1, SULT1C4 and ABCC2 genes were genotyped. A significant (p < 0.05) relationship between the SNPs in these genes and the high excretion of phase II flavanone metabolites were observed. These results identified novel polymorphisms associated with higher absorption of flavanones, which may provide bases for future personalized nutritional guidelines for consuming flavanone-rich foods rich in these nutrients for better benefit from their health properties.

Keywords: ABC transporters; SNP; bioavailability; flavanone metabolite; hesperidin; interindividual variability; narirutin; orange juice; polymorphism; sulfotransferase.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Total phase II flavanone metabolite concentrations in urine depending on genotypes of ABCC2_rs8187710 polymorphism. Bars in the box show the first quartile, the third quartile and median and whisker charts represent the minimum to maximum values.
Figure 2
Figure 2
Total metabolite concentrations in urine depending on genotypes of SULT1A1_rs3760091 polymorphism. Bars in the box show the first quartile, the third quartile, and median and whisker charts represent the minimum to maximum values.
Figure 3
Figure 3
Total metabolite concentrations in urine depending on genotypes of SULT1A1_rs4788068 (B) polymorphism. Bars in the box show the first quartile, the third quartile, and median and whisker charts represent the minimum to maximum values.
Figure 4
Figure 4
Total metabolite concentrations in urine depending on genotypes of SULT1C4_rs1402467 polymorphism. Bars in the box show the first quartile, the third quartile, and median and whisker charts represent the minimum to maximum values.
Figure 5
Figure 5
Total metabolite concentrations in urine depending the GGCC and GCTG haplotypes for ABCC2_rs8187710/SULT1A1_rs3760091/SULT1A1_rs4788068/SULT1C4_rs1402467 with phenotype high excretors.
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