Docosahexaenoic acid-loaded chitosan/alginate membrane reduces biofilm formation by P. aeruginosa and promotes MSC-mediated burn wound healing
- PMID: 36189675
- DOI: 10.1177/08853282221131130
Docosahexaenoic acid-loaded chitosan/alginate membrane reduces biofilm formation by P. aeruginosa and promotes MSC-mediated burn wound healing
Abstract
Aims: Chitosan, like docosahexaenoic acid (DHA) and mesenchymal stem cells (MSCs), is used in medicine as a wound healing accelerator. Thus, in this study, chitosan-alginate (CA) membranes containing DHA and MSCs were produced, and their antibacterial and antibiofilm activities against burn infections caused by Pseudomonas aeruginosa were investigated.Methods: Physicochemical properties were assessed by SEM, Fourier transform infrared (FTIR), and X-ray diffraction (XRD). Porosity, cytocompatibility, and antibacterial and antibiofilm activities were evaluated both in vitro and in vivo. The viability and apoptosis of MSCs were studied using flow cytometry. Wound healing effects were analyzed based on histopathological features, the wound contraction rate (WCR) ratio, and bacterial clearance.Results: The CA membranes showed antibiofilm activity both in vivo and in vitro, accompanied by reduced lasI and rhlI expressions and pyocyanin production. The membranes were highly porous and biocompatible and showed favorable physicochemical properties. Docosahexaenoic acid incorporation to CA membranes improved their antibacterial and antibiofilm activities, as well as MSCs' viability by reducing crystallinity and increasing porosity (p = .008). Treatment with CA-DHA-MSC accelerated burn wound healing (with complete healing being observed after 14 days, WCR = 85%) and augmented antibacterial and antibiofilm activities in vivo compared to CA-DHA and CA-MSC. The CA-DHA-MSC group delivered a significantly higher WCR and lower inflammation than the CA-MSC group (p = .0001).Conclusion: In combination with DHA-loaded CA membranes, MSCs reduced the healing time of burn wounds, offering a viable option for designing effective wound dressings.
Keywords: Mesenchymal stem cells; antibiofilm; burn wound healing; chitosan; docosahexaenoic acid; pseudomonas aeruginosa.
Similar articles
-
How the combination of alginate and chitosan can fabricate a hydrogel with favorable properties for wound healing.Heliyon. 2024 Jun 3;10(11):e32040. doi: 10.1016/j.heliyon.2024.e32040. eCollection 2024 Jun 15. Heliyon. 2024. PMID: 38912439 Free PMC article. Review.
-
Cu-MOF loaded chitosan based freeze-dried highly porous dressings with anti-biofilm and pro-angiogenic activities accelerated Pseudomonas aeruginosa infected wounds healing in rats.Int J Biol Macromol. 2024 Jun;271(Pt 2):132443. doi: 10.1016/j.ijbiomac.2024.132443. Epub 2024 May 16. Int J Biol Macromol. 2024. PMID: 38761913
-
Potential antibacterial activity and healing effect of topical administration of bone marrow and adipose mesenchymal stem cells encapsulated in collagen-fibrin hydrogel scaffold on full-thickness burn wound infection caused by Pseudomonas aeruginosa.Burns. 2023 Dec;49(8):1944-1957. doi: 10.1016/j.burns.2023.01.005. Epub 2023 Jan 16. Burns. 2023. PMID: 38042618
-
Electrospun Nanofibrous Biocomposite of Royal Jelly/Chitosan/Polyvinyl Alcohol (RJ/CS/PVA) Gel as a Biological Dressing for P. aeruginosa-Infected Burn Wound.Appl Biochem Biotechnol. 2024 Jun;196(6):3162-3183. doi: 10.1007/s12010-023-04701-9. Epub 2023 Aug 26. Appl Biochem Biotechnol. 2024. PMID: 37632660
-
Mesenchymal stem cell-based therapy for burn wound healing.Burns Trauma. 2021 May 1;9:tkab002. doi: 10.1093/burnst/tkab002. eCollection 2021. Burns Trauma. 2021. PMID: 34212055 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical