Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Dec;35(6):1359-1370.
doi: 10.1007/s10534-022-00451-z. Epub 2022 Oct 20.

Molecular characterization and expression analysis of selenoprotein W gene in rainbow trout (Oncorhynchus mykiss) with dietary selenium levels

Chenlei Liao  1 , Feng Zhang  1 , Zhenlei Teng  1 , Guirong Zhang  1 , Ying Yang  1 , Pengke Xu  1 , Xixuan Huang  1 , Li Wang  1 , Fan Yang  2 , Zhilong Yang  2 , Xuezhen Zhang  3
Affiliations

Molecular characterization and expression analysis of selenoprotein W gene in rainbow trout (Oncorhynchus mykiss) with dietary selenium levels

Chenlei Liao et al. Biometals. 2022 Dec.

Abstract

Selenium (Se) plays an essential role in the growth of fish and performs its physiological functions mainly through incorporation into selenoproteins. Our previous studies suggested that the selenoprotein W gene (selenow) is sensitive to changes in dietary Se in rainbow trout. However, the molecular characterization and tissue expression pattern of selenow are still unknown. Here, we revealed the molecular characterization, the tissue expression pattern of rainbow trout selenow and analyzed its response to dietary Se. The open reading frame (ORF) of the selenow gene was composed of 393 base pairs (bp) and encodes a 130-amino-acid protein. The 3' untranslated region (UTR) was 372 bp with a selenocysteine insertion sequence (SECIS) element. Remarkably, the rainbow trout selenow gene sequence was longer than those reported for mammals and most other fish. A β1-α1-β2-β3-β4-α2 pattern made up the secondary structure of SELENOW. Furthermore, multiple sequence alignment revealed that rainbow trout SELENOW showed a high level of identity with SELENOW from Salmo salar. In addition, the selenow gene was ubiquitously distributed in 13 tissues with various abundances and was predominantly expressed in muscle and brain. Interestingly, dietary Se significantly increased selenow mRNA expression in muscle. Our results highlight the vital role of selenow in rainbow trout muscle response to dietary Se levels and provide a theoretical basis for studies of selenow.

Keywords: Dietary selenium; Gene expression; Rainbow trout; Selenow; cDNA cloning.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Aachmann FL, Fomenko DE, Soragni A, Gladyshev VN, Dikiy A (2007) Solution structure of selenoprotein W and NMR analysis of its interaction with 14–3-3 proteins. J Biol Chem 282:37036–37044. https://doi.org/10.1074/jbc.M705410200 - DOI - PubMed
    1. Beilstein MA, Vendeland SC, Barofsky E, Jensen ON, Whanger PD (1996) Selenoprotein W of rat muscle binds glutathione and an unknown small molecular weight moiety. J Inorg Biochem 61:117–124. https://doi.org/10.1016/0162-0134(95)00045-3 - DOI - PubMed
    1. Chambers I, Frampton J, Goldfarb P, Affara N, McBain W, Harrison PR (1986) The structure of the mouse glutathione peroxidase gene: the selenocysteine in the active site is encoded by the ‘termination’ codon, TGA. EMBO J 5:1221–1227. https://doi.org/10.1002/j.1460-2075.1986.tb04350.x - DOI - PubMed - PMC
    1. Chapple CE, Guigó R, Krol A (2009) SECISaln, a web-based tool for the creation of structure-based alignments of eukaryotic SECIS elements. Bioinformatics 25:674–675. https://doi.org/10.1093/bioinformatics/btp020 - DOI - PubMed - PMC
    1. Chen W, Zhang Z, Dong H, Jiang X (2015) Molecular cloning and sequence analysis of selenoprotein W gene and its mRNA expression patterns in response to metabolic status and cadmium exposure in goldfish, Carassius auratus. Comp Biochem Physiol B 184:1–9. https://doi.org/10.1016/j.cbpb.2015.01.005 - DOI - PubMed

Publication types

LinkOut - more resources

-