Culture and Differentiation of Primary Preadipocytes from Mouse Subcutaneous White Adipose Tissue
- PMID: 37076667
- PMCID: PMC10583291
- DOI: 10.1007/978-1-0716-3167-6_2
Culture and Differentiation of Primary Preadipocytes from Mouse Subcutaneous White Adipose Tissue
Abstract
Adipocytes are terminally differentiated cells derived from fibroblastic preadipocyte precursors. Here, we describe a method for the isolation and proliferation of preadipocytes from murine subcutaneous white adipose tissue, followed by differentiation in culture to mature adipocytes; we refer to these cells as primary preadipocytes differentiated in vitro (PPDIVs). Compared to adipogenic cell lines, PPDIV metabolism and adipokine secretion more closely resemble in vivo adipocyte biology. While primary mature adipocytes have the greatest in vivo relevance, their fragility and buoyancy make them unsuitable for many cell culture-based methods. PPDIVs can also take advantage of transgenic and knockout mouse models to produce genetically modified adipocytes. Thus, PPDIVs are a valuable resource for studying adipocyte biology in cell culture.
Keywords: Adipogenesis; Collagenase; Differentiation; Lipid droplet; Primary preadipocyte; Primary preadipocyte differentiated in vitro (PPDIV); Stromal vascular fraction (SVF); White adipocyte; White adipose tissue (WAT).
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Figures
![Fig. 1](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/10583291/bin/nihms-1935586-f0001.gif)
![Fig. 2](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/10583291/bin/nihms-1935586-f0002.gif)
![Fig. 3](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/10583291/bin/nihms-1935586-f0003.gif)
Similar articles
-
Adipose tissue engineering in three-dimensional levitation tissue culture system based on magnetic nanoparticles.Tissue Eng Part C Methods. 2013 May;19(5):336-44. doi: 10.1089/ten.TEC.2012.0198. Epub 2012 Nov 2. Tissue Eng Part C Methods. 2013. PMID: 23017116 Free PMC article.
-
Isolation and study of adipocyte precursors.Methods Enzymol. 2014;537:31-46. doi: 10.1016/B978-0-12-411619-1.00003-3. Methods Enzymol. 2014. PMID: 24480340 Free PMC article.
-
Technical note: Bovine adipocyte and preadipocyte co-culture as an efficient adipogenic model.J Dairy Sci. 2019 Apr;102(4):3622-3629. doi: 10.3168/jds.2018-15626. Epub 2019 Feb 14. J Dairy Sci. 2019. PMID: 30772027
-
Cellular models for understanding adipogenesis, adipose dysfunction, and obesity.J Cell Biochem. 2010 Jun 1;110(3):564-72. doi: 10.1002/jcb.22598. J Cell Biochem. 2010. PMID: 20512917 Review.
-
In vitro and ex vivo models of adipocytes.Am J Physiol Cell Physiol. 2021 May 1;320(5):C822-C841. doi: 10.1152/ajpcell.00519.2020. Epub 2021 Jan 13. Am J Physiol Cell Physiol. 2021. PMID: 33439778 Review.
Cited by
-
Measuring the Rate of Lipolysis in Ex vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro.J Vis Exp. 2023 Mar 17;(193):10.3791/65106. doi: 10.3791/65106. J Vis Exp. 2023. PMID: 37010285 Free PMC article.
References
-
- Rodbell M (1964) Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J Biol Chem 239:375–380 - PubMed
-
- Bjorntorp P, Karlsson M, Pertoft H, Pettersson P, Sjostrom L, Smith U (1978) Isolation and characterization of cells from rat adipose tissue developing into adipocytes. J Lipid Res 19(3):316–324 - PubMed
Publication types
MeSH terms
Grants and funding
LinkOut - more resources
Full Text Sources