Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models
- PMID: 37328521
- PMCID: PMC10275883
- DOI: 10.1038/s41597-023-02293-x
Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models
Abstract
Adipogenesis is a process in which fat-specific progenitor cells (preadipocytes) differentiate into adipocytes that carry out the key metabolic functions of the adipose tissue, including glucose uptake, energy storage, and adipokine secretion. Several cell lines are routinely used to study the molecular regulation of adipogenesis, in particular the immortalized mouse 3T3-L1 line and the primary human Simpson-Golabi-Behmel syndrome (SGBS) line. However, the cell-to-cell variability of transcriptional changes prior to and during adipogenesis in these models is not well understood. Here, we present a single-cell RNA-Sequencing (scRNA-Seq) dataset collected before and during adipogenic differentiation of 3T3-L1 and SGBS cells. To minimize the effects of experimental variation, we mixed 3T3-L1 and SGBS cells and used computational analysis to demultiplex transcriptomes of mouse and human cells. In both models, adipogenesis results in the appearance of three cell clusters, corresponding to preadipocytes, early and mature adipocytes. These data provide a groundwork for comparative studies on these widely used in vitro models of human and mouse adipogenesis, and on cell-to-cell variability during this process.
© 2023. The Author(s).
Conflict of interest statement
The authors declare no competing interests.
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Update of
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Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models.bioRxiv [Preprint]. 2023 Mar 29:2023.03.27.534456. doi: 10.1101/2023.03.27.534456. bioRxiv. 2023. Update in: Sci Data. 2023 Jun 16;10(1):387. doi: 10.1038/s41597-023-02293-x. PMID: 37034809 Free PMC article. Updated. Preprint.
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