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. 2023:34:69-102.
doi: 10.1007/978-3-031-36159-3_2.

Techniques to Render Dendritic Spines Visible in the Microscope

Floris G Wouterlood  1
Affiliations

Techniques to Render Dendritic Spines Visible in the Microscope

Floris G Wouterlood. Adv Neurobiol. 2023.

Abstract

A tiny detail visible on certain neurons at the limit of resolution in light microscopy went in 130 years of neuroscience research through a dazzling career from suspicious staining artifact to what we recognize today as a complex postsynaptic molecular machine: the dendritic spine.This chapter deals with techniques to make spines visible. The original technique, Golgi silver staining, is still being used today. Electron microscopy and automated field ion beam scanning electron microscopy are ultrahigh resolution techniques, albeit specialized. Other methods are intracellular injection, uptake of dyes, and recently the exploitation of genetically modified animals in which certain neurons express fluorescent protein in all their processes, including the nooks and crannies of their dendritic spines.

Keywords: 3D reconstruction; Carbocyanine dyes; FIB-SEM; Genetic engineering; Golgi silver impregnation; Intracellular injection; Microscopy.

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