Development of a Holistic In Vitro Cell-Free Approach to Determine the Redox Bioactivity of Agricultural Products
- PMID: 38003634
- PMCID: PMC10671064
- DOI: 10.3390/ijms242216447
Development of a Holistic In Vitro Cell-Free Approach to Determine the Redox Bioactivity of Agricultural Products
Abstract
In recent years, there has been a strong consumer demand for food products that provide nutritional benefits to human health. Therefore, the assessment of the biological activity is considered as an important parameter for the promotion of high-quality food products. Herein, we introduce a novel methodology comprising a complete set of in vitro cell-free screening techniques for the evaluation of the bioactivity of various food products on the basis of their antioxidant capacity. These assays examine the free radical scavenging activities, the reducing properties, and the protective ability against oxidative damage to biomolecules. The adoption of the proposed battery of antioxidant assays is anticipated to contribute to the holistic characterization of the bioactivity of the food product under examination. Consumer motivations and expectations with respect to nutritious food products with bio-functional properties drive the global food market toward food certification. Therefore, the development and application of scientific methodologies that examine the quality characteristics of food products could increase consumers' trust and promote their beneficial properties for human health.
Keywords: agricultural products; antioxidant capacity; bioactivity; cell-free assays; methodology.
Conflict of interest statement
The authors declare no conflict of interest.
Similar articles
-
Influencing Factors and Group Differences of Urban Consumers' Willingness to Pay for Low-Carbon Agricultural Products in China.Int J Environ Res Public Health. 2022 Dec 26;20(1):358. doi: 10.3390/ijerph20010358. Int J Environ Res Public Health. 2022. PMID: 36612679 Free PMC article.
-
When food governance matters to consumer food choice: Consumer perception of and preference for food quality certifications.Appetite. 2022 Jan 1;168:105688. doi: 10.1016/j.appet.2021.105688. Epub 2021 Sep 9. Appetite. 2022. PMID: 34509543
-
Functional Food-Consumer Motivations and Expectations.Int J Environ Res Public Health. 2021 May 17;18(10):5327. doi: 10.3390/ijerph18105327. Int J Environ Res Public Health. 2021. PMID: 34067768 Free PMC article. Review.
-
A novel combined bioactivity / chemoactivity holistic approach for the evaluation of dietary supplements.Food Chem Toxicol. 2021 Jun;152:112159. doi: 10.1016/j.fct.2021.112159. Epub 2021 Mar 28. Food Chem Toxicol. 2021. PMID: 33789120
-
The credibility of the effects of functional food products and consumers' willingness to purchase/willingness to pay- review.Appetite. 2019 Dec 1;143:104398. doi: 10.1016/j.appet.2019.104398. Epub 2019 Aug 8. Appetite. 2019. PMID: 31401236 Review.
References
-
- Paniker N., Srivastava S., Beutler E. Glutathione metabolism of the red cells effect of glutathione reductase deficiency on the stimulation of hexose monophosphate shunt under oxidative stress. Biochim. Biophys. Acta (BBA)-Gen. Subj. 1970;215:456–460. doi: 10.1016/0304-4165(70)90096-6. - DOI - PubMed
-
- Sies H. Oxidative stress: Eustress and distress in redox homeostasis. In: Fink G., editor. Stress, Physiology, Biochemistry, and Pathology. Volume 3. Academic Press; Cambridge, MA, USA: 2019. pp. 153–163. - DOI
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical