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Protein-lipid interactions drive presynaptic assembly upstream of cell adhesion molecules
- PMID: 38014115
- PMCID: PMC10680821
- DOI: 10.1101/2023.11.17.567618
Protein-lipid interactions drive presynaptic assembly upstream of cell adhesion molecules
Abstract
Textbook models of synaptogenesis position cell adhesion molecules such as neurexin as initiators of synapse assembly. Here we discover a mechanism for presynaptic assembly that occurs prior to neurexin recruitment, while supporting a role for neurexin in synapse maintenance. We find that the cytosolic active zone scaffold SYD-1 interacts with membrane phospholipids to promote active zone protein clustering at the plasma membrane, and subsequently recruits neurexin to stabilize those clusters. Employing molecular dynamics simulations to model intrinsic interactions between SYD-1 and lipid bilayers followed by in vivo tests of these predictions, we find that PIP2-interacting residues in SYD-1's C2 and PDZ domains are redundantly necessary for proper active zone assembly. Finally, we propose that the uncharacterized yet evolutionarily conserved short γ isoform of neurexin represents a minimal neurexin sequence that can stabilize previously assembled presynaptic clusters, potentially a core function of this critical protein.
Conflict of interest statement
DECLARATION OF COMPETING INTERESTS The authors declare no competing interests.
Figures
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