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. 2024 Apr 3;11(4):163.
doi: 10.3390/vetsci11040163.

Use of Ozone in Veterinary Dentistry as an Alternative to Conventional Antibiotics and Antiseptics

Pierre Melanie  1 , Carlotta Niola  2 , Ilaria Plataroti  3 , Simone Mancini  1   4 , Filippo Fratini  1   4
Affiliations

Use of Ozone in Veterinary Dentistry as an Alternative to Conventional Antibiotics and Antiseptics

Pierre Melanie et al. Vet Sci. .

Abstract

This paper aims to assess the disinfecting capacity of a double-distilled ozonated water solution as an alternative to common antibiotic and antiseptic devices. Seventy-five dogs were subjected to the surgical procedures of scaling and dental extraction and included in three study groups: Group 1 subjected to antibiotic pre-treatment (association amoxicillin + clavulanic acid and ampicillin + metronidazole) and disinfection with chlorhexidine, and Group 2 and 3 devoid of preventive anti-microbial treatment in which disinfection was performed, respectively, with ozonated water and chlorhexidine. Sampling by bacteriological buffer was carried out to evaluate the bacterial count in the oral cavity. The analysis of the samples determined the total mesophilic bacterial count by seeding on the culture medium via the inclusion of PCA (Plate Count Agar). The results highlighted the anti-bacterial efficacy of intra-operative ozone as an alternative to pre-surgical antibiotic treatment.

Keywords: anti-bacterial; dogs; microbiology; ozonated water; periodontal pathology; scaling.

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Conflict of interest statement

The authors declare no conflicts of interest that may be perceived as inappropriately influencing the representation or interpretation of reported research results.

Figures

Figure 1
Figure 1
A graphic representation of the outcomes in each treatment group. Bacteriological swabs were taken at: Group 1—pre-treatment phase (T0), post-antibiotic phase (T1), and post-treatment phase (T2); Group 2 pre-treatment phase (T0) and post-treatment phase (T2); Group 3 pre-treatment phase (T0) and post-treatment phase (T2).
Figure 2
Figure 2
Graphical representation of the variability in pre-treatment phase (T0) and post-antibiotic phase (T1) among each subject in Group 1.
See this image and copyright information in PMC

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References

    1. Wallis C., Holcombe L.J. A review of the frequency and impact of periodontal disease in dogs. J. Small Anim. Pract. 2020;61:529–540. doi: 10.1111/jsap.13218. - DOI - PubMed
    1. Chen W.-P., Chang S.-H., Tang C.-Y., Liou M.-L., Tsai S.-J.J., Lin Y.-L. Composition Analysis and Feature Selection of the Oral Microbiota Associated with Periodontal Disease. BioMed Res. Int. 2018;2018:3130607. doi: 10.1155/2018/3130607. - DOI - PMC - PubMed
    1. Santibáñez R., Rodríguez-Salas C., Flores-Yáñez C., Garrido D., Thomson P. Assessment of Changes in the Oral Microbiome That Occur in Dogs with Periodontal Disease. Vet. Sci. 2021;8:291. doi: 10.3390/vetsci8120291. - DOI - PMC - PubMed
    1. Logan E.I. Dietary influences on periodontal health in dogs and cats. Vet. Clin. N. Am. Small Anim. Pract. 2006;36:1385–1401. doi: 10.1016/j.cvsm.2006.09.002. - DOI - PubMed
    1. Yamaki S., Tachibana M., Hachimura H., Ogawa M., Kanegae S., Amimoto H., Shimizu T., Watanabe K., Watarai M., Amimoto A. The association between gingivitis and oral spirochetes in young cats and dogs. PLoS ONE. 2023;18:e0281126. doi: 10.1371/journal.pone.0281126. - DOI - PMC - PubMed

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