A method to analyze gene expression profiles from hippocampal neurons electrophysiologically recorded in vivo
- PMID: 38694898
- PMCID: PMC11061373
- DOI: 10.3389/fnins.2024.1360432
A method to analyze gene expression profiles from hippocampal neurons electrophysiologically recorded in vivo
Abstract
Hippocampal pyramidal neurons exhibit diverse spike patterns and gene expression profiles. However, their relationships with single neurons are not fully understood. In this study, we designed an electrophysiology-based experimental procedure to identify gene expression profiles using RNA sequencing of single hippocampal pyramidal neurons whose spike patterns were recorded in living mice. This technique involves a sequence of experiments consisting of in vivo juxtacellular recording and labeling, brain slicing, cell collection, and transcriptome analysis. We demonstrated that the expression levels of a subset of genes in individual hippocampal pyramidal neurons were significantly correlated with their spike burstiness, submillisecond-level spike rise times or spike rates, directly measured by in vivo electrophysiological recordings. Because this methodological approach can be applied across a wide range of brain regions, it is expected to contribute to studies on various neuronal heterogeneities to understand how physiological spike patterns are associated with gene expression profiles.
Keywords: burstiness; firing rate; juxtacellular recording; single cell RNA sequencing; spike rise time.
Copyright © 2024 Yagishita, Go, Okamoto, Arimura, Ikegaya and Sasaki.
Conflict of interest statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that NA and TS were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.
Figures
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