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. 2024 May 11;70(1):6.
doi: 10.1007/s00294-024-01290-w.

Hypothesis: evidence that the PRS gene products of Saccharomyces cerevisiae support both PRPP synthesis and maintenance of cell wall integrity

Emily Murdoch  1 , Lilian M Schweizer  2 , Michael Schweizer  3
Affiliations

Hypothesis: evidence that the PRS gene products of Saccharomyces cerevisiae support both PRPP synthesis and maintenance of cell wall integrity

Emily Murdoch et al. Curr Genet. .

Abstract

The gene products of PRS1-PRS5 in Saccharomyces cerevisiae are responsible for the production of PRPP (5-phospho-D-ribosyl-α-1-pyrophosphate). However, it has been demonstrated that they are also involved in the cell wall integrity (CWI) signalling pathway as shown by protein-protein interactions (PPIs) with, for example Slt2, the MAP kinase of the CWI pathway. The following databases: SGD, BioGRID and Hit Predict, which collate PPIs from various research papers, have been scrutinized for evidence of PPIs between Prs1-Prs5 and components of the CWI pathway. The level of certainty in PPIs was verified by interaction scores available in the Hit Predict database revealing that well-documented interactions correspond with higher interaction scores and can be graded as high confidence interactions based on a score > 0.28, an annotation score ≥ 0.5 and a method-based high confidence score level of ≥ 0.485. Each of the Prs1-Prs5 polypeptides shows some degree of interaction with the CWI pathway. However, Prs5 has a vital role in the expression of FKS2 and Rlm1, previously only documented by reporter assay studies. This report emphasizes the importance of investigating interactions using more than one approach since every method has its limitations and the use of different methods, as described herein, provides complementary experimental and statistical data, thereby corroborating PPIs. Since the experimental data described so far are consistent with a link between PRPP synthetase and the CWI pathway, our aim was to demonstrate that these data are also supported by high-throughput bioinformatic analyses promoting our hypothesis that two of the five PRS-encoding genes contain information required for the maintenance of CWI by combining data from our targeted approach with relevant, unbiased data from high-throughput analyses.

Keywords: CWI signalling; Gene duplication; Non-homologous regions; PRPP synthetase; Targeted database screening.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Physical Prs protein-protein Interactions (PPIs) and Prs/CWI pathway protein interactions identified from Hit Predict with corresponding interaction scores. (A) Prs1 PPIs, (B) Prs2 PPIs, (C) Prs3 PPIs, (D) Prs4 PPIs and (E) Prs5 PPIs. Segment size varies and is dependent on the number of proteins the Prs protein interacts with, and the computed interaction score. The size of the segment is directly proportional to the interaction. A greater interaction score = a larger segment. An interaction score > 0.28 is deemed high confidence. Data were extracted from Hit Predict (http://www.hitpredict.org/index.html)
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