Rapid induction of the expression of proto-oncogene fos during human monocytic differentiation
- PMID: 3881183
- DOI: 10.1016/0092-8674(85)90324-1
Rapid induction of the expression of proto-oncogene fos during human monocytic differentiation
Abstract
The differentiation into macrophages of the monocytic cell line U-937 or the monomyelocytic cell line HL-60 induced by phorbol esters is accompanied by a rapid induction of the expression of the proto-oncogene c-fos. Maximal levels of c-fos gene transcripts accumulate within 20-30 min following induction. By 2 hr, the c-fos-specific mRNA level drops 5 to 10 fold below that detected at 30 min after induction. The concentration of c-fos mRNA remains unchanged for the next 5 days, at which time over 99% of the viable precursor cells are converted to adherent macrophages. c-fos protein can be detected within 20 min of induction, reaching maximal levels by 40-60 min. Little or no c-fos protein is detected by 120-150 min after induction, even though c-fos mRNA remains detectable. Presence of cycloheximide leads to superinduction of c-fos mRNA transcripts. No c-fos gene transcripts are detected when the HL-60 cell line is induced with Me2SO to differentiate to granulocytes. A role for the c-fos protein is envisaged in the monocyte differentiation pathway.
Similar articles
-
Differences in expression of transcription factor AP-1 in human promyelocytic HL-60 cells during differentiation towards macrophages versus granulocytes.Biochem J. 1993 Aug 15;294 ( Pt 1)(Pt 1):137-44. doi: 10.1042/bj2940137. Biochem J. 1993. PMID: 8363564 Free PMC article.
-
Transient induction of c-fos and c-myc in an immediate consequence of growth factor stimulation.Cancer Surv. 1985;4(4):655-81. Cancer Surv. 1985. PMID: 3939686 Review.
-
Induction of c-sis gene expression and synthesis of platelet-derived growth factor in human myeloid leukemia cells during monocytic differentiation.Proc Natl Acad Sci U S A. 1986 Sep;83(17):6455-9. doi: 10.1073/pnas.83.17.6455. Proc Natl Acad Sci U S A. 1986. PMID: 3529084 Free PMC article.
-
c-fos expression is neither sufficient nor obligatory for differentiation of monomyelocytes to macrophages.Cell. 1986 May 23;45(4):497-504. doi: 10.1016/0092-8674(86)90281-3. Cell. 1986. PMID: 3085953
-
Transcriptional and post-transcriptional regulation of c-jun expression during monocytic differentiation of human myeloid leukemic cells.J Biol Chem. 1990 Feb 25;265(6):3320-3. J Biol Chem. 1990. PMID: 2105946
Cited by
-
Prenylation differentially inhibits insulin-dependent immediate early gene mRNA expression.Biochem Biophys Res Commun. 2016 Jun 3;474(3):594-598. doi: 10.1016/j.bbrc.2016.04.067. Epub 2016 Apr 14. Biochem Biophys Res Commun. 2016. PMID: 27086854 Free PMC article.
-
AFos dissociates cardiac myocyte hypertrophy and expression of the pathological gene program.Circulation. 2005 Apr 5;111(13):1645-51. doi: 10.1161/01.CIR.0000160367.99928.87. Epub 2005 Mar 28. Circulation. 2005. PMID: 15795322 Free PMC article.
-
Isolation of a mammalian homologue of a fission yeast differentiation regulator.Mol Cell Biol. 1999 May;19(5):3829-41. doi: 10.1128/MCB.19.5.3829. Mol Cell Biol. 1999. PMID: 10207106 Free PMC article.
-
Rodent cell transformation and immediate early gene expression following 60-Hz magnetic field exposure.Environ Health Perspect. 1996 Nov;104(11):1188-98. doi: 10.1289/ehp.961041188. Environ Health Perspect. 1996. PMID: 8959408 Free PMC article.
-
Exon 2-mediated c-myc mRNA decay in vivo is independent of its translation.Mol Cell Biol. 1996 Sep;16(9):5107-16. doi: 10.1128/MCB.16.9.5107. Mol Cell Biol. 1996. PMID: 8756668 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources