Plant regeneration in the new era: from molecular mechanisms to biotechnology applications

doi:10.1007/s11427-024-2581-2

Review

. 2024 Jul;67(7):1338-1367.

doi: 10.1007/s11427-024-2581-2. Epub 2024 May 31.

Plant regeneration in the new era: from molecular mechanisms to biotechnology applications

Chunli Chen^{1

2}, Yuxin Hu³, Momoko Ikeuchi⁴, Yuling Jiao^{5

6}, Kalika Prasad^{7

8}, Ying Hua Su^{9

10}, Jun Xiao^{11

12}, Lin Xu¹³, Weibing Yang^{14

15}, Zhong Zhao¹⁶, Wenkun Zhou¹⁷, Yun Zhou¹⁸, Jian Gao¹⁹, Jia-Wei Wang^{20

21

22

23}

Affiliations

¹ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China. chenchunli@mail.hzau.edu.cn.
² College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. chenchunli@mail.hzau.edu.cn.
³ Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences (CAS), China National Botanical Garden, Beijing, 100093, China. huyuxin@ibcas.ac.cn.
⁴ Division of Biological Sciences, Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara, 630-0192, Japan. momoko.ikeuchi@bs.naist.jp.
⁵ State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China. yuling.jiao@pku.edu.cn.
⁶ Peking-Tsinghua Center for Life Sciences, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China. yuling.jiao@pku.edu.cn.
⁷ Indian Institute of Science Education and Research, Pune, 411008, India. kalika.prasad@iiserpune.ac.in.
⁸ , Thiruvananthapuram, 695551, India. kalika.prasad@iiserpune.ac.in.
⁹ State Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China. suyh@sdau.edu.cn.
¹⁰ Sino-German Joint Research Center on Agricultural Biology, Shandong Agricultural University, Tai'an, 271018, China. suyh@sdau.edu.cn.
¹¹ Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology (IGDB), CAS, Beijing, 100101, China. jxiao@genetics.ac.cn.
¹² CAS-JIC Centre of Excellence for Plant and Microbial Science (CEPAMS), IGDB, CAS, Beijing, 100101, China. jxiao@genetics.ac.cn.
¹³ National Key Laboratory of Plant Molecular Genetics, CEMPS, Institute of Plant Physiology and Ecology (SIPPE), CAS, Shanghai, 200032, China. xulin@cemps.ac.cn.
¹⁴ National Key Laboratory of Plant Molecular Genetics, CEMPS, Institute of Plant Physiology and Ecology (SIPPE), CAS, Shanghai, 200032, China. wbyang@cemps.ac.cn.
¹⁵ CEPAMS, SIPPE, CAS, Shanghai, 200032, China. wbyang@cemps.ac.cn.
¹⁶ Hefei National Laboratory for Physical Sciences at the Microscale, CEMPS, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, China. zhzhao@ustc.edu.cn.
¹⁷ State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. zhouwenkun@cau.edu.cn.
¹⁸ Department of Botany and Plant Pathology and Center for Plant Biology, Purdue University, West Lafayette, 47907, USA. zhouyun@purdue.edu.
¹⁹ National Key Laboratory of Plant Molecular Genetics, CEMPS, Institute of Plant Physiology and Ecology (SIPPE), CAS, Shanghai, 200032, China.
²⁰ National Key Laboratory of Plant Molecular Genetics, CEMPS, Institute of Plant Physiology and Ecology (SIPPE), CAS, Shanghai, 200032, China. jwwang@sippe.ac.cn.
²¹ School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China. jwwang@sippe.ac.cn.
²² Key Laboratory of Plant Carbon Capture, CAS, Shanghai, 200032, China. jwwang@sippe.ac.cn.
²³ New Cornerstone Science Laboratory, Shanghai, 200032, China. jwwang@sippe.ac.cn.

PMID: 38833085
DOI: 10.1007/s11427-024-2581-2

Review

Plant regeneration in the new era: from molecular mechanisms to biotechnology applications

Chunli Chen et al. Sci China Life Sci. 2024 Jul.

. 2024 Jul;67(7):1338-1367.

doi: 10.1007/s11427-024-2581-2. Epub 2024 May 31.

Authors

Affiliations

¹ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China. chenchunli@mail.hzau.edu.cn.
² College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. chenchunli@mail.hzau.edu.cn.
³ Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences (CAS), China National Botanical Garden, Beijing, 100093, China. huyuxin@ibcas.ac.cn.
⁴ Division of Biological Sciences, Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara, 630-0192, Japan. momoko.ikeuchi@bs.naist.jp.
⁵ State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China. yuling.jiao@pku.edu.cn.
⁶ Peking-Tsinghua Center for Life Sciences, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China. yuling.jiao@pku.edu.cn.
⁷ Indian Institute of Science Education and Research, Pune, 411008, India. kalika.prasad@iiserpune.ac.in.
⁸ , Thiruvananthapuram, 695551, India. kalika.prasad@iiserpune.ac.in.
⁹ State Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China. suyh@sdau.edu.cn.
¹⁰ Sino-German Joint Research Center on Agricultural Biology, Shandong Agricultural University, Tai'an, 271018, China. suyh@sdau.edu.cn.
¹¹ Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology (IGDB), CAS, Beijing, 100101, China. jxiao@genetics.ac.cn.
¹² CAS-JIC Centre of Excellence for Plant and Microbial Science (CEPAMS), IGDB, CAS, Beijing, 100101, China. jxiao@genetics.ac.cn.
¹³ National Key Laboratory of Plant Molecular Genetics, CEMPS, Institute of Plant Physiology and Ecology (SIPPE), CAS, Shanghai, 200032, China. xulin@cemps.ac.cn.
¹⁴ National Key Laboratory of Plant Molecular Genetics, CEMPS, Institute of Plant Physiology and Ecology (SIPPE), CAS, Shanghai, 200032, China. wbyang@cemps.ac.cn.
¹⁵ CEPAMS, SIPPE, CAS, Shanghai, 200032, China. wbyang@cemps.ac.cn.
¹⁶ Hefei National Laboratory for Physical Sciences at the Microscale, CEMPS, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, China. zhzhao@ustc.edu.cn.
¹⁷ State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. zhouwenkun@cau.edu.cn.
¹⁸ Department of Botany and Plant Pathology and Center for Plant Biology, Purdue University, West Lafayette, 47907, USA. zhouyun@purdue.edu.
¹⁹ National Key Laboratory of Plant Molecular Genetics, CEMPS, Institute of Plant Physiology and Ecology (SIPPE), CAS, Shanghai, 200032, China.
²⁰ National Key Laboratory of Plant Molecular Genetics, CEMPS, Institute of Plant Physiology and Ecology (SIPPE), CAS, Shanghai, 200032, China. jwwang@sippe.ac.cn.
²¹ School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China. jwwang@sippe.ac.cn.
²² Key Laboratory of Plant Carbon Capture, CAS, Shanghai, 200032, China. jwwang@sippe.ac.cn.
²³ New Cornerstone Science Laboratory, Shanghai, 200032, China. jwwang@sippe.ac.cn.

PMID: 38833085
DOI: 10.1007/s11427-024-2581-2

Abstract

Plants or tissues can be regenerated through various pathways. Like animal regeneration, cell totipotency and pluripotency are the molecular basis of plant regeneration. Detailed systematic studies on Arabidopsis thaliana gradually unravel the fundamental mechanisms and principles underlying plant regeneration. Specifically, plant hormones, cell division, epigenetic remodeling, and transcription factors play crucial roles in reprogramming somatic cells and reestablishing meristematic cells. Recent research on basal non-vascular plants and monocot crops has revealed that plant regeneration differs among species, with various plant species using distinct mechanisms and displaying significant differences in regenerative capacity. Conducting multi-omics studies at the single-cell level, tracking plant regeneration processes in real-time, and deciphering the natural variation in regenerative capacity will ultimately help understand the essence of plant regeneration, improve crop regeneration efficiency, and contribute to future crop design.

Keywords: apical meristem; model species; phytohormone; plant cell reprogramming; regeneration; somatic embryogenesis.

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References

1. Abe, T., and Futsuhara, Y. (1986). Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.). Theoret Appl Genet 72, 3–10. - DOI
1. Adamczyk, B.J., Lehti-Shiu, M.D., and Fernandez, D.E. (2007). The MADS domain factors AGL15 and AGL18 act redundantly as repressors of the floral transition in Arabidopsis. Plant J 50, 1007–1019. - PubMed - DOI
1. Aichinger, E., Kornet, N., Friedrich, T., and Laux, T. (2012). Plant stem cell niches. Annu Rev Plant Biol 63, 615–636. - PubMed - DOI
1. Aichinger, E., Villar, C.B.R., Di Mambro, R., Sabatini, S., and Köhler, C. (2011). The CHD3 chromatin remodeler PICKLE and polycomb group proteins antagonistically regulate meristem activity in the Arabidopsis root. Plant Cell 23, 1047–1060. - PubMed - PMC - DOI
1. Aida, M., Beis, D., Heidstra, R., Willemsen, V., Blilou, I., Galinha, C., Nussaume, L., Noh, Y.S., Amasino, R., and Scheres, B. (2004). The PLETHORA genes mediate patterning of the Arabidopsis root stem cell niche. Cell 119, 109–120. - PubMed - DOI

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[1] Abe, T., and Futsuhara, Y. (1986). Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.). Theoret Appl Genet 72, 3–10. - DOI

[2] Abe, T., and Futsuhara, Y. (1986). Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.). Theoret Appl Genet 72, 3–10. - DOI

[3] Adamczyk, B.J., Lehti-Shiu, M.D., and Fernandez, D.E. (2007). The MADS domain factors AGL15 and AGL18 act redundantly as repressors of the floral transition in Arabidopsis. Plant J 50, 1007–1019. - PubMed - DOI

[4] Adamczyk, B.J., Lehti-Shiu, M.D., and Fernandez, D.E. (2007). The MADS domain factors AGL15 and AGL18 act redundantly as repressors of the floral transition in Arabidopsis. Plant J 50, 1007–1019. - PubMed - DOI

[5] Aichinger, E., Kornet, N., Friedrich, T., and Laux, T. (2012). Plant stem cell niches. Annu Rev Plant Biol 63, 615–636. - PubMed - DOI

[6] Aichinger, E., Kornet, N., Friedrich, T., and Laux, T. (2012). Plant stem cell niches. Annu Rev Plant Biol 63, 615–636. - PubMed - DOI

[7] Aichinger, E., Villar, C.B.R., Di Mambro, R., Sabatini, S., and Köhler, C. (2011). The CHD3 chromatin remodeler PICKLE and polycomb group proteins antagonistically regulate meristem activity in the Arabidopsis root. Plant Cell 23, 1047–1060. - PubMed - PMC - DOI

[8] Aichinger, E., Villar, C.B.R., Di Mambro, R., Sabatini, S., and Köhler, C. (2011). The CHD3 chromatin remodeler PICKLE and polycomb group proteins antagonistically regulate meristem activity in the Arabidopsis root. Plant Cell 23, 1047–1060. - PubMed - PMC - DOI

[9] Aida, M., Beis, D., Heidstra, R., Willemsen, V., Blilou, I., Galinha, C., Nussaume, L., Noh, Y.S., Amasino, R., and Scheres, B. (2004). The PLETHORA genes mediate patterning of the Arabidopsis root stem cell niche. Cell 119, 109–120. - PubMed - DOI

[10] Aida, M., Beis, D., Heidstra, R., Willemsen, V., Blilou, I., Galinha, C., Nussaume, L., Noh, Y.S., Amasino, R., and Scheres, B. (2004). The PLETHORA genes mediate patterning of the Arabidopsis root stem cell niche. Cell 119, 109–120. - PubMed - DOI

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Plant regeneration in the new era: from molecular mechanisms to biotechnology applications

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Plant regeneration in the new era: from molecular mechanisms to biotechnology applications

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