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. 2024 Jun;59(6):e14648.
doi: 10.1111/rda.14648.

Quality and fertilizing ability of frozen-thawed porcine sperm separated using a migration sedimentation method

Suong Thi Nguyen  1   2 , Masayasu Taniguchi  1 , Tetsushi Ono  1 , Mitsuhiro Takagi  1 , Qingyi Lin  2 , Nanaka Torigoe  2 , Bin Liu  2 , Zhao Namula  2   3 , Megumi Nagahara  2 , Takeshige Otoi  2
Affiliations

Quality and fertilizing ability of frozen-thawed porcine sperm separated using a migration sedimentation method

Suong Thi Nguyen et al. Reprod Domest Anim. 2024 Jun.

Abstract

We evaluated the quality and fertilizing ability of frozen-thawed porcine sperm that were selected using a commercially available device (MIGLIS, Menicon Life Science) consisting of three parts: an outer lid, an inner lid, and a tube. Firstly, to determine an adequate concentration of caffeine for separation, frozen-thawed sperm were incubated with different concentrations of caffeine (0, 1, 2.5, 5, and 10 mM) in a MIGLIS device. To determine the appropriate incubation time for separating sperm in the MIGLIS device, frozen-thawed sperm were incubated with 2.5 mM caffeine for 5, 10, 15, or 20 min. To evaluate the fertilization and embryo development of oocytes fertilized with frozen-thawed sperm separated into two regions (outer and inner) in the MIGLIS device, the separated sperm from the three boars was used to fertilize in vitro-matured oocytes and cultured in vitro for 7 days. Sperm quality parameters of sperm collected from the inner tube after incubation with 2.5 mM caffeine were superior to sperm incubated without caffeine. Moreover, sperm collected from the inner tube after incubation for 10 min had a higher progressive motility. The rate of blastocyst produced from spermatozoa collected from the inner tube after incubation with 2.5 mM caffeine for 10 min significantly increased compared to that produced from spermatozoa from the outer tube, regardless of the boar. In conclusion, sperm sorting using the MIGLIS device may be useful for separating high-quality sperm after incubation with 2.5 mM caffeine for 10 min to improve blastocyst formation.

Keywords: MIGLIS; boar; caffeine; in vitro fertilization; sperm.

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References

REFERENCES

    1. Abeydeera, L. R. (2002). In vitro production of embryos in swine. Theriogenology, 57(1), 256–273. https://doi.org/10.1016/s0093‐691x(01)00670‐7
    1. Ahmad, Z., Anzar, M., Shahab, M., Ahmad, N., & Andrabi, S. M. (2003). Sephadex and Sephadex ion‐exchange filtration improves the quality and freezability of low‐grade buffalo semen ejaculates. Theriogenology, 59(5–6), 1189–1202. https://doi.org/10.1016/s0093‐691x(02)01159‐7
    1. Alves, N. C., Diniz, S. A., Viegas, R. N., Arigoni, A. L., Freitas, M. M., Lana, Â. Q., & Lagares, M. A. (2022). Action of swim‐up and caffeine on equine frozen sperm. Animal Reproduction, 19(4), e20220056. https://doi.org/10.1590/1984‐3143‐AR2022‐0056
    1. Alves, N. C., Diniz, S. A., Viegas, R. N., Cortes, S. F., Costa, E. D., Freitas, M. M., Martins‐Filho, O. A., Araújo, M. S. S., Lana, Â. M. Q., Wenceslau, R. R., & Lagares, M. A. (2021). Addition of caffeine to equine thawed sperm increases motility and decreases nitrite concentration. Andrologia, 53(2), e13918. https://doi.org/10.1111/and.13918
    1. Banihani, S. A., & Khaled, H. J. (2021). Caffeine increased progressive motility of human spermatozoa in normozoospermic and asthenozoospermic semen samples and enhanced activity of seminal creatine kinase. Andrologia, 53(6), e14052. https://doi.org/10.1111/and.14052

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