Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jun 24;206(7):324.
doi: 10.1007/s00203-024-04059-4.

Citral and triclosan synergistically silence quorum sensing and potentiate antivirulence response in Pseudomonas aeruginosa

Jatin Chadha #  1 , Prerna Ahuja #  1 , Umang Mudgil  1 , Lavanya Khullar  1 , Kusum Harjai  2
Affiliations

Citral and triclosan synergistically silence quorum sensing and potentiate antivirulence response in Pseudomonas aeruginosa

Jatin Chadha et al. Arch Microbiol. .

Abstract

Among the ESKAPE pathogens, Pseudomonas aeruginosa is an extensively notorious superbug that causes difficult-to-treat infections. Since quorum sensing (QS) directly promotes pseudomonal virulence, targeting QS circuits is a promising approach for disarming phenotypic virulence. Hence, this study scrutinizes the anti-QS, antivirulence, and anti-biofilm potential of citral (CiT; phytochemical) and triclosan (TcN; disinfectant), alone and in combination, against P. aeruginosa PAO1/PA14. The findings confirmed synergism between CiT and TcN and revealed their quorum quenching (QQ) potential. At sub-inhibitory levels, CiT-TcN combination significantly impeded pyocyanin, total bacterial protease, hemolysin, and pyochelin production alongside inhibiting biofilm formation in P. aeruginosa. Moreover, the QQ and antivirulence potential of CiT and TcN was positively correlated by molecular docking studies that predicted strong associations of the drugs with QS receptors of P. aeruginosa. Collectively, the study identifies CiT-TcN as an effective drug combination that harbors QQ, antivirulence, and anti-biofilm prospects against P. aeruginosa.

Keywords: Biofilm inhibition; Citral; Pseudomonas aeruginosa; Quorum quenching; Triclosan; Virulence.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Ajao C et al (2015) Mitochondrial toxicity of triclosan on mammalian cells. Toxicol Rep 2:624–637. https://doi.org/10.1016/j.toxrep.2015.03.012 - DOI - PubMed - PMC
    1. Batohi N, Lone SA, Marimani M, Wani MY, Al-Bogami AS, Ahmad A (2021) Citral and its derivatives inhibit quorum sensing and biofilm formation in Chromobacterium violaceum. Arch Microbiol 203:1451–1459. https://doi.org/10.1007/s00203-020-02127-z - DOI - PubMed
    1. Bose SK, Chauhan M, Dhingra N, Chhibber S, Harjai K (2020) Terpinen-4-ol attenuates quorum sensing regulated virulence factors and biofilm formation in Pseudomonas aeruginosa. Future Microbiol 15:127–142. https://doi.org/10.2217/fmb-2019-0204 - DOI - PubMed
    1. Cao Y, Zhou D, Zhang X, Xiao X, Yu Y, Li X (2021) Synergistic effect of citral and carvacrol and their combination with mild heat against Cronobacter sakazakii CICC 21544 in reconstituted infant formula. Lwt 138:110617. https://doi.org/10.1016/j.lwt.2020.110617 - DOI
    1. Chadha J (2021) In vitro effects of sub-inhibitory concentrations of amoxicillin on physiological responses and virulence determinants in a commensal strain of Escherichia coli. J Appl Microbiol 131:682–694. https://doi.org/10.1111/jam.14987 - DOI - PubMed

MeSH terms

LinkOut - more resources

-