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. 2024:2810:75-83.
doi: 10.1007/978-1-0716-3878-1_5.

Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor

Jenny Gunnarsson  1 , David I Fisher  2 , Robert G Roth  3
Affiliations

Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor

Jenny Gunnarsson et al. Methods Mol Biol. 2024.

Abstract

Large culture volumes are often required when expression constructs are particularly low-yielding or when end uses require significant amounts of material. In these cases, a single homogeneous culture is usually more convenient, in terms of both consistency of expression and labor/resource requirements, than multiple parallel cultures. Using a WAVE Bioreactor culture, volumes as high as 500L may be achieved in a single vessel. Here, we describe the transfection of Expi293F cells in a disposable 50L Cellbag on a WAVE Bioreactor platform to produce recombinant protein. The methods described herein may be adapted, with suitable optimizations, for other suspension-adapted mammalian cell lines.

Keywords: Cell culture; Mammalian; Polyethylenimine; Recombinant protein; Transient transfection; WAVE Bioreactor.

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