Assessing Transcriptomic Responses to Oxidative Stress: Contrasting Wild-Type Arabidopsis Seedlings with dss1(I) and dss1(V) Gene Knockout Mutants
- PMID: 38927997
- PMCID: PMC11203560
- DOI: 10.3390/ijms25126291
Assessing Transcriptomic Responses to Oxidative Stress: Contrasting Wild-Type Arabidopsis Seedlings with dss1(I) and dss1(V) Gene Knockout Mutants
Abstract
Oxidative stress represents a critical facet of the array of abiotic stresses affecting crop growth and yield. In this paper, we investigated the potential differences in the functions of two highly homologous Arabidopsis DSS1 proteins in terms of maintaining genome integrity and response to oxidative stress. In the context of homologous recombination (HR), it was shown that overexpressing AtDSS1(I) using a functional complementation test increases the resistance of the Δdss1 mutant of Ustilago maydis to genotoxic agents. This indicates its conserved role in DNA repair via HR. To investigate the global transcriptome changes occurring in dss1 plant mutant lines, gene expression analysis was conducted using Illumina RNA sequencing technology. Individual RNA libraries were constructed from three total RNA samples isolated from dss1(I), dss1(V), and wild-type (WT) plants under hydrogen peroxide-induced stress. RNA-Seq data analysis and real-time PCR identification revealed major changes in gene expression between mutant lines and WT, while the dss1(I) and dss1(V) mutant lines exhibited analogous transcription profiles. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed significantly enriched metabolic pathways. Notably, genes associated with HR were upregulated in dss1 mutants compared to the WT. Otherwise, genes of the metabolic pathway responsible for the synthesis of secondary metabolites were downregulated in both dss1 mutant lines. These findings highlight the importance of understanding the molecular mechanisms of plant responses to oxidative stress.
Keywords: Arabidopsis thaliana; AtDSS1; CRISPR/Cas9 plant mutants; RNA-seq; Ustilago maydis; homologous recombination; oxidative stress.
Similar articles
-
CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response.Int J Mol Sci. 2023 Jan 26;24(3):2442. doi: 10.3390/ijms24032442. Int J Mol Sci. 2023. PMID: 36768765 Free PMC article.
-
Intrinsically disordered protein AtDSS1(V) participates in plant defense response to oxidative stress.Protoplasma. 2021 Jul;258(4):779-792. doi: 10.1007/s00709-020-01598-7. Epub 2021 Jan 6. Protoplasma. 2021. PMID: 33404921
-
Loss of Arabidopsis Halotolerance 2-like (AHL), a 3'-phosphoadenosine-5'-phosphate phosphatase, suppresses insensitive response of Arabidopsis thaliana ring zinc finger 1 (atrzf1) mutant to abiotic stress.Plant Mol Biol. 2019 Mar;99(4-5):363-377. doi: 10.1007/s11103-019-00822-0. Epub 2019 Jan 12. Plant Mol Biol. 2019. PMID: 30637572
-
Salt hypersensitive mutant 9, a nucleolar APUM23 protein, is essential for salt sensitivity in association with the ABA signaling pathway in Arabidopsis.BMC Plant Biol. 2018 Mar 1;18(1):40. doi: 10.1186/s12870-018-1255-z. BMC Plant Biol. 2018. PMID: 29490615 Free PMC article.
-
Cytokinin Response Factor 6 Represses Cytokinin-Associated Genes during Oxidative Stress.Plant Physiol. 2016 Oct;172(2):1249-1258. doi: 10.1104/pp.16.00415. Epub 2016 Aug 22. Plant Physiol. 2016. PMID: 27550996 Free PMC article.
References
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources