Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jun 20;16(6):991.
doi: 10.3390/v16060991.

Development of a Ferritin Protein Nanoparticle Vaccine with PRRSV GP5 Protein

Xinjian Chang  1   2 , Jun Ma  1   2 , Yanrong Zhou  1   2 , Shaobo Xiao  1   2 , Xun Xiao  1   2 , Liurong Fang  1   2
Affiliations
Free PMC article

Development of a Ferritin Protein Nanoparticle Vaccine with PRRSV GP5 Protein

Xinjian Chang et al. Viruses. .
Free PMC article

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) presents a significant threat to the global swine industry. The development of highly effective subunit nanovaccines is a promising strategy for preventing PRRSV variant infections. In this study, two different types of ferritin (Ft) nanovaccines targeting the major glycoprotein GP5, named GP5m-Ft and (Bp-IVp)3-Ft, were constructed and evaluated as vaccine candidates for PRRSV. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) demonstrated that both purified GP5m-Ft and (Bp-IVp)3-Ft proteins could self-assemble into nanospheres. A comparison of the immunogenicity of GP5m-Ft and (Bp-IVp)3-Ft with an inactivated PRRSV vaccine in BALB/c mice revealed that mice immunized with GP5m-Ft exhibited the highest ELISA antibody levels, neutralizing antibody titers, the lymphocyte proliferation index, and IFN-γ levels. Furthermore, vaccination with the GP5m-Ft nanoparticle effectively protected piglets against a highly pathogenic PRRSV challenge. These findings suggest that GP5m-Ft is a promising vaccine candidate for controlling PRRS.

Keywords: PRRSV; ferritin; subunit vaccine; virus-like particle.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Vet Res. 2013 Mar 11;44:17 - PubMed
    1. Trop Anim Health Prod. 2016 Oct;48(7):1351-9 - PubMed
    1. J Virol. 2002 May;76(9):4241-50 - PubMed
    1. Pharmaceutics. 2021 Oct 05;13(10): - PubMed
    1. Mol Biotechnol. 2015 Aug;57(8):701-8 - PubMed

MeSH terms

LinkOut - more resources

-