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. 1969 Jan;16(1):35-43.

Glutaraldehyde as a coupling reagent in passive haemagglutination

E Onkelinx, W Meuldermans, M Joniau, R Lontie

Glutaraldehyde as a coupling reagent in passive haemagglutination

E Onkelinx et al. Immunology. 1969 Jan.

Abstract

A sensitive passive haemagglutination technique is described depending on the attachment of proteins to erythrocytes with glutaraldehyde. Various factors that play a role in the coupling of bovine serum albumin to sheep erythrocytes are examined. A standard procedure was deduced by which other proteins were coupled and tested with their specific antisera. The observed titres are comparable to those obtained with the classical bis-diazotized benzidine method.

The technique is specific and practicable. It provides sensitized cells that remain stable for several weeks.

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References

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