Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Aug;18(2):358-64.
doi: 10.1128/jcm.18.2.358-364.1983.

Antigenic relationships among some bovine rotaviruses: serum neutralization and cross-protection in gnotobiotic calves

G N Woode, N E Kelso, T F Simpson, S K Gaul, L E Evans, L Babiuk

Antigenic relationships among some bovine rotaviruses: serum neutralization and cross-protection in gnotobiotic calves

G N Woode et al. J Clin Microbiol. 1983 Aug.

Abstract

A method was further developed to screen non-tissue-culture-adapted bovine rotaviruses for serotype, using a neutralization test with infectious fecal rotavirus. One of those rotaviruses (B223) which was not blocked by antiserum to the neonatal calf diarrhea virus (NCDV) serotype was then adapted to cell culture in the presence of the antiserum for two or more passages and hyperimmune antiserum to this isolate had a 60-fold-higher homologous neutralization titer than with the NCDV serotype rotavirus. Seventy-three isolates were serotyped and eight (11%) were not of the NCDV serotype (bovine rotavirus serotype I). Of these eight, five belonged to the new bovine rotavirus serotype II and three were not typed, indicating the existence of one or more further serotypes. Cross-protection studies in gnotobiotic calves showed that cross-protection only occurred between rotaviruses of the same serotype, and even a minor serotype difference was sufficient for the calves to show a lack of cross-protection. The serotypes (I and II and the three untyped isolates) also showed differences in the rate of migration in polyacrylamide gel electrophoresis of some of their RNA segments (no. 4, 6, 7, 8, 9, 10), indicating that they were of different electropherotypes.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Can J Comp Med. 1971 Jul;35(3):195-202 - PubMed
    1. Res Vet Sci. 1974 Jan;16(1):102-5 - PubMed
    1. Vet Pathol. 1976;13(3):197-210 - PubMed
    1. Arch Virol. 1977;53(4):287-94 - PubMed

Publication types

LinkOut - more resources

-