Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 May;81(9):2616-20.
doi: 10.1073/pnas.81.9.2616.

DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication

S DiNardo, K Voelkel, R Sternglanz

DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication

S DiNardo et al. Proc Natl Acad Sci U S A. 1984 May.

Abstract

A temperature-sensitive DNA topoisomerase II mutant of the yeast Saccharomyces cerevisiae has been identified. Genetic analysis shows that a single recessive nuclear mutation is responsible for both temperature-sensitive growth and enzymatic activity. Thus, topoisomerase II is essential for viability and the mutation is most probably in the structural gene. Experiments with synchronized mutant cells show that at the nonpermissive temperature cells can undergo one, and only one, round of DNA replication. These cells are arrested at medial nuclear division. Analysis of 2-microns plasmid DNA from these cells shows it to be in the form of multiply intertwined catenated dimers. The results suggest that DNA topoisomerase II is necessary for the segregation of chromosomes at the termination of DNA replication.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Bacteriol. 1967 May;93(5):1662-70 - PubMed
    1. Cell. 1983 Apr;32(4):1313-24 - PubMed
    1. J Bacteriol. 1973 Sep;115(3):966-74 - PubMed
    1. Exp Cell Res. 1973 Nov;82(1):47-56 - PubMed
    1. Eur J Biochem. 1974 Jan 16;41(2):359-65 - PubMed

Publication types

Substances

LinkOut - more resources

-