doi: 10.1093/nar/11.9.2575.
Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides
- PMID: 6856470
- PMCID: PMC325909
- DOI: 10.1093/nar/11.9.2575
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Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides
Nucleic Acids Res.
.
Free PMC article
Abstract
The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reactions during solid-phase synthesis can thus be performed with high efficiency and result in a high yield synthesis of long chain oligodeoxyribonucleotides.
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